I. Abstract In this experiment, the concentrations of Allura Red in three beverages were found. To do this, five Allura Red solutions were made and their absorbances were used to construct a calibration curve. Then, 7-Up, Gatorade, and Powerade solutions were created and analyzed in a spectrophotometer. The concentrations of Allura Red in each beverage were 0.11 mM for Gatorade, 0.10 mM for Powerade, and 0.0074 mM for 7-Up. Finally, the mass of Allura Red in each beverage and the limit to how many bottles of each beverage are safe to consume daily were determined.
II. Introduction Allura Red is a dye that…show more content… Graph 1 -. Calibration curve constructed using absorbances presented in Table S1 with a slope of 25 mM-1 and a y-intercept of 0.0273. Experimental molar absorptivity Map: (Slope (mM-1) x 1000 mM/M) / Path length (cm) Work: (25.4 mM-1 x 1000 mM/M) / 1.00 cm = 25,400 M-1 cm-1.
Average absorbances of diluted beverages Map: (Absorbance 1 + Absorbance 2 + Absorbance 3) / 3 Work (7-Up): (0.199 + 0.203 + 0.237) / 3 = 0.213 Work (Powerade): (0.504 + 0.522 + 0.544) / 3 = 0.523 Work (Gatorade): (0.561 + 0.535 + 0.570) / 3 = 0.555 Work (Gatorade): (0.561 + 0.535 + 0.570) / 3 =
Concentrations of dye in diluted beverages Map: (Average absorbance – y intercept) / Slope Work (7-Up): (0.213 – 0.0273) / 25 mM-1 = 0.0074 mM Work (Powerade): (0.523 – 0.0273) / 25 mM-1 = 0.020 mM Work (Gatorade): (0.555 – 0.0273) / 25 mM-1 = 0.021 mM Work
