Amylase And Starch Lab Report

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Purpose-
The purpose of this experiment is to see how chemical reactions can be sped up using enzymes under different conditions.

Procedure-
Please refer to the “Enzymes-The Catalysts of Life” Lab (Flinn Scientific), pages 2-3 for the proper procedure.

Data- see attached sheet

Graph- see attached sheet

Results and Discussion-
Effects of Amylase on Starch: In this experiment, 4 test tubes were tested with benedict’s solution. This solution will turn the substance orange if sugars are present. One tube was filled with starch, another filled with glucose, one containing starch and the enzyme, amylase, and finally a tube filled with distilled water to be used as a control because water would not change color. An additional 3 tubes …show more content…
It was understood that the amylase should have broken the starch down into sugar, causing the iodine test for starch to come out negative. However, in the experiment, the tube still tested positive for starch. This may have been caused by adding too much starch, or not allowing the amylase enough time to break the starch down.

Effects of Amylase Concentration on Starch In part 2 of this experiment, starch was added to each of twelve wells on a reaction plate. A 10% concentration of amylase was added to each well and tested for starch using iodine. The experiment was then repeated with 20%, 30%, 40%, and 50% amylase, with the goal of discovering the impact that the amylase concentration had on the performance of the enzyme. When tested, it showed that the higher the concentration, the less the enzyme worked and the more wells tested positive. However, this is not what should have been found. The higher concentration of amylase should have broken the starch down and caused the wells to test negative. Likewise, the lower concentrations should have worked less effectively, causing the wells to test positive for starch. Contamination of the plate and inaccurate droppings may have
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