ASEPTIC TECHNIQUE AND BACTERIAL ANATOMY AND MORPHOLOGY
Introduction
Part I: Aseptic Technique
The purpose of this experiment is to become familiar with the specific microbiological technique known as the aseptic technique, which is used to avoid contaminating cultures.
In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complaining to his doctor about pain in his lower abdominal and back. With a family history of prostate cancer, he worried that his sudden need to urinate during the night and infrequent appearance of blood in his urine might be bad signs. An ultrasound…show more content… Record the results.
Click and drag the media over the biohazard container to dispose.
Part II: Bacterial Anatomy
Sample 1
Click the New button and select Bacterial Anatomy 1 from the Select Exercise Topic or Subgroup dropdown box.
Type Sample 1 Lab #6 in the Enter Unknown Identifier field.
Click the box allowing autoinoculation.
Observe the Gram reaction of the unknown and indicate the results of the gram stain by selecting the result that pertains to the observation and record the result by clicking record gram reaction.
Sample 2
Click the New button and select Bacterial Anatomy 2 from the Select Exercise Topic or Subgroup dropdown box.
Type Sample 2 Lab #6 in the Enter Unknown Identifier field.
Click the box allowing autoinoculation.
Observe the Gram reaction of the unknown and indicate the results of the gram stain by selecting the result that pertains to the observation and record the result by clicking record gram reaction.
Observations and Results
Part I: Aseptic Technique
Gram stain result: Gram negative rod.
Part 4: * Amount of time it took until the Contamination traffic light turned red: 2 minutes. * Appearance of tube after overnight incubation: the color changed from red to yellow.
Part 5: * My aseptic transfer was successful. The inoculation and contamination lights were green. * Appearance of tube after overnight incubation: the color
