Temp.
Potential Inaccurate measurements: egg cube cutter not accurate cubes meaning different volumes of cubes (not all 5mm by 5mm)
Temperature of water in the beaker wasn’t stable due to the external environment.
The 5ml of HCL was not accurate with the pipette as it was difficult to grasp the needed amount.
The 10ml of Pepsin solution “ “
Using a stopwatch was more accurate as opposed to counting.
Improvements that could have been made:
Kept the water stable in the beaker by adding ice or adding heated water
Indication on ‘mL’ wasn’t clear perhaps using a more precise tool such as a ‘burette’.
To ensure experiment is accurate; through reliability, repeat the experiment keeping all variables the same.
Measure each egg cube to ensure they all have the same volume and dimensions. (5mm by 5mm)
The results did not support the hypothesis, as there was no evidence of the egg white turning colourless at 40 degrees.
Validity: the experiment correctly tests the aim, as it did investigate the changing temperatures on the breakdown of egg albumen but didn’t support the hypothesis, therefore It’s not valid, also not valid due to the lack of accuracy and the many minor errors that occurred in the experiment.
Not reliable, as it wasn’t repeated.
Not accurate, measurements weren’t precise.
pH
Potential inaccurate measurements: egg cube cutter not accurate cubes meaning different volumes of cubes (not all 5mm by 5mm) the 400mL beaker with the 200mL water was not stable at 40 degrees due to external environment.
Pepsin may not have ben entirely dissolved in the distilled water
Measurements with the HCL pH wasn’t accurate with the pipette pH of each solutions were incorrect
Due to the little range of HCL pH levels we were forced to use pH1 and pH2 and distilled water (ph7)
Improvements that could have been made:
Kept the water stable in the beaker by adding ice or adding heated water
Indication on ‘mL’ wasn’t clear perhaps using a more precise tool such as a ‘burette’.
To ensure experiment is accurate; through reliability, repeat the experiment keeping all variables the same.
Measure each egg cube to ensure they all have the same volume and dimensions. (5mm by 5mm)
The results did support the hypothesis as it did have the fastest rate of reaction at pH level 2 and similarly at pH level 1. (in terms of flakiness )
Validity: The experiment correctly tests the aim, as it did investigate the changing pH levels on the enzyme pepsin with the substrate egg white. It did support the hypothesis but not valid due to the lack of accuracy and the many minor errors that occurred in the experiment.
Not reliable, as it wasn’t repeated.
Not accurate, measurements weren’t precise.
Enzyme Concentration
Potential inaccurate measurements:
Egg cube cutter not accurate cubes meaning different volumes of cubes (not all 5mm by 5mm)