Chemical: Chromatography and Green Food Dye Essay

Submitted By joshuabeehag
Words: 381
Pages: 2
Open Document
CHEMICAL SEPERATIONS Abstract
This report outlines numerous techniques used to separate and purify chemicals. During the study crystals, sand and hot water were dissolved, separated then crystallized to form crystals. Thin layer and column Chromatography was used to separate and identify compounds in spinach and green food dye.
Introduction
Since ancient times, people have used methods of separating and purifying chemical substances for improving the quality of life. There are a large number of important applications and numerous techniques that can be used. (Karger 2014 ). This paper discusses 3 techniques used to demonstrate the separation and purification of chemicals. Recrystallization was performed in order to recover crystals of copper sulphate pentahydrate by filtering copper sulphate from a mixture of sand and salt. Thin Layer Chromatography of spinach extract focussed on determining the spectrum of chlorophylls, carotenes and xanthophylls. Column Chromatography of green food dye was used to visually and spectrometrically determine whether the dye is one green component or a mixture of blue and yellow components. The results remain consistent with previous experiments, within experimental error.

Materials and Methods
Methods for Part A – Recrystallization of a

Results
Part A
Mass of recrystallisation mixture = 10g
Description of the mixture: contains grains of crystals sand and hot water
Notes and observations on the dissolution: crystals dissolved on contact with hot water. Sand was left and a blue colour
Show More

Related Documents: Chemical: Chromatography and Green Food Dye Essay

Ap Chem Food Dye Chromatography Lab

AP Chem Food Dye Chromatography Minilab Name: Ben Wong Background: Chromatography is a lab technique often used to separate out a mixture of substances using the concept “like dissolves like”, meaning things with similar polarity and intermolecular forces will dissolve in each other. There are two main components in a chromatography experiment: the stationary phase and the mobile phase. The stationary phase is a piece of paper that is stained with the chemicals one is trying to separate. The…

Words 1290 - Pages 6

Spectrophotometry: Technique To Determine The Chemical Composition And Concentration Of Lab

Biology Lab Study Guide: Labs 1-6 Lab #2: Spectrophotometry: Technique to determine the chemical composition and concentration of sample. The spectrophotometer: instrument that measures the amount of light transmitted by the sample. ~ 5 key components: a) Light Source = produces white light, combination of all visible wavelengths b) Monochromator (prism shaped)=separates the light c) Subject= sample solution being tested d) Photo detector = converts light energy into…

Words 1372 - Pages 6

Lycopene Research Paper

radicals in our body include chronic diseases, smoking, environmental poisons, alcohol and ionizing radiation. Overproduction of free radicals can result in oxidative stress, a deleterious process that damages the cell structure. Antioxidants are chemical compounds that can bind to free oxygen radicals preventing these radicals from damaging healthy cells whereas pro-antioxidant act indirectly either by modulation of direct agents or by regulation of the biosynthesis of antioxidant proteins. (Nurul…

Words 3430 - Pages 14