Chromatography Lab Report

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Because most substances are colorless, the separation caused by thin-layer chromatography can go unnoticed until the compound is visualized. If fluorescent plates are used, the number and distance traveled of compounds can be seen by illuminating the plate with ultraviolet light (Saferstein, 2011). The materials on the plate will appear as bright spots on a dark background, where they can then be circled with pencil. For molecules that are not activated by UV light, the chemical stain iodine proves effective for visualization. Treated plates are placed in a jar with iodine crystals, staining all organic compounds brown (UC Davis Chemistry Department, 2013). While simplicity and cost-effectiveness lend themselves to the popularity of thin-layer …show more content…
Similar to capillary action chromatography, components of varying polarities will travel down the column at different rates; because molecules belonging to the same compound will travel together, the compounds are separated into distinct bands of colors within the glass column, as evident in Figure 3 (UC Boulder, 2015). If the solvent moves down the column by air pressure instead of gravity, it is known as flash chromatography. Column chromatography was the brainchild of Russian botanist Mikhail Tswett, who was able to separate plant pigments using glass columns filled with calcium carbonate (New World Encyclopedia, 2013). Then in 1978, Clark Still introduced a modification to column chromatography that was faster and more efficient than gravity-fed chromatography, claiming it could be "done in a flash" (UC Boulder, 2015). It differs from the conventional technique because it uses 10-15 psi from an attached air pump to drive the solvent through the column, allowing separation that used to take days to be made in hours (New World Encyclopedia,

Chromatography Lab Report

INTRODUCTION CHROMATOGRAPHY Chromatography was originally developed in the year 1903 by the Russian botanist Michael Tswett in percolating a petroleum ether extract through a glass column packed with powered calcium carbonate for the separation of colored pigments. Elution means a chromatographic separation involves the placing of the sample into a liquid or solid stationary phase and passing a liquid or gaseous mobile phase through or over it. Whether…

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Chromatography Lab Report

Procedure: Part C: First, obtain the unknown test tube that has dissolved pigment. Determine what color pigment you want to target and look use to qualitative observations from part A/B in order to find a way to determine what metal ion is likely to be inside the test tube. Since white pigment has either zinc or lead, you can target these by testing potassium iodide and record the observations, then compare them to the observations made from part A. First, obtain a 4mL test tube, and fill it with…

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Gas Chromatography Lab Report

compound makes up in a given a mixture. Distillation is the process of heating a liquid to boiling and then collecting the condensed vapors. With these condensed vapors, a gas chromatography can be employed, to determine the relative portion of a compound in a given mixture. This por-tion is shown as a percent area on a gas chromatography graph. This percent area is important as it helps explain the physical properties, such as boiling point of a mixture. When observing the percent area of all the fractions…

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Paper Chromatography Lab Report

Paper Chromatography Compare and Contrast By: Karson Raak Upon comparing our group’s paper chromatography samples with other groups, there are several things that become apparent. First, is a strip of red Sharpie from our group compared to a strip of red Sharpie from the other group, both of which were suspended in ethanol. In the interests of solubility, the red Sharpie was not as soluble as the other markers. Both tests exhibit the ink to not move much at all. Where it does move, it quickly becomes…

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Lab Report Thin Layer Chromatography

1.Objective-Our goal for this experiment is to perform thin layer and column chromatography. What chromatography does is separate colored mixtures of substances. 2.Introduction- We most understand how to do both types of Chromatography. In thin layer chromatography the plate is the stationary phase and the movement phase is the Toluene. In column chromatography a packed column is the stationary phase and the movement phase is the ethanol and water. Also one equation that we will be using is r1=D1/Ds…

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Thin Layer Chromatography Lab Report

Thin Layer Chromatography Date performed: 21/01/13 Date submitted: Introduction: The objective of the experiment is to find out the identity of the unknown sample provided in powder form. The goal was to check its content and find its identity by consulting the table provided in the book. There were total three possible drugs present namely caffeine, aspirin and acetaminophen. Each drug in the table has a different percentage of these substances. TLC i-e Thin layer chromatography is based…

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Thin Layer Chromatography Lab Report

Thin Layer Chromatography (TLC) is a technique of chromatography where compounds are separated from a mixture using an absorbent material on a structural sheet or plate. There are many uses for TLC, one use of this experiment is to answer how many components are in a mixture. When the TLC plate is placed in a mixture, the material on the plate absorbs the mixture in the beaker. The compounds spotted on the plate move along with the solvent via capillary action. The farther the spots move up the TLC…

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Chromatography Lab Report: Use The Same Decay For Red Candies

Chromatography Lab Report Through the course of the Chromatography lab, the question trying to be answered was “do Skittles™ and M&M’s™ use the same dye for red candies?” The reason for conducting this experiment is to find out whether or not the two brands of candies use the same dye. The hypothesis derived from this question is if the two candies are made with the same dye, then each lane of dye and solvent will travel the same distance because the dyes are the same size and polarity. The dependent…

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Thin Layer Chromatography Lab Report Essay

INTRODUCTION The purpose of this experiment was to identify the two bodies recovered from separate lakes because the bodies were mixed up at the morgue using the Thin Layer Chromatography (TLC) method. TLC is a technique for separating dissolved chemical substances by virtue of their migration over plastic sheets coated with a thin layer of finely grounded adsorbent silica gel (Encyclopaedia Britannica. Britannica Academic, 2015). It is to support the identity of a compound in a mixture when the…

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TLC-Thin Layer Chromatography Lab Report

In TLC, chromatography was run on 7 samples, methyl salicylate, standard salicylic acid, standard acetylsalicylic acid, crude salicylic acid, purified salicylic acid, crude acetylsalicylic acid and purified acetylsalicylic acid. Chromatography is used to separate and identify mixtures of different compounds. The procedure we use to identify the compounds is by comparing the…

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