Using Wnt1 and Sox10 as Markers for Craniofacial Osteosarcoma Tumor Cells for Retainment of Neural Crest Stem Cells
Lillian Zhang | Dr. Marc Hansen, University of Connecticut, School of Medicine
Period 3 | January 17, 2017
Abstract
Using Wnt1 and Sox10 as Markers for Craniofacial Osteosarcoma Tumor Cells for Retainment of Neural Crest Stem Cells
Osteosarcoma (OS) is the most common form of primary bone cancer. Primary craniofacial OS tumors form in areas of the skull that are derived from the embryonic rests of the neural crest due to the malignant transformation of neural crest stem cells (NCSCs). Previous research suggests some stem cells still remain in the jawbone. The purpose of the study is to compare expression patterns of craniofacial OS tumors and stem cells to better understand how OS tumors arise from NCSCs. The cells were labeled immunohistochemically using an antibody against markers Wnt1 and Sox10, captured using laser capture microdissection to isolate populations and then compared. The control (human jaw bone) and the NCSCs (of the young mouse jaws of 8.5, 10.5, and 16.5 day embryos) were compared to each other…show more content… During surgical resection, tumor margins are identified so the whole tumor can be removed in its encapsulated perimeter. In positive surgical margins, the whole tumor may not be completely removed. This can be detrimental for the patient if not noticed, because the remaining tumor cells can multiply and grow to an extent. By using wide-excision, surgeons will be able to remove the complete tumor if a layer of normal cells are removed as well. In 50% of cases, complete surgical resection is limited by the extension of tumors into nearby areas. Thus, it is important to correctly identify tumor margins in order to improve survival rates in OS tumors
