daphnia heart rate Essay example

Submitted By Jesus-Anaya
Words: 1542
Pages: 7
Open Document
An Investigation to Show the Effect of Temperature on Daphnia
Plan:
I will submerge some daphnia contained in a test tube in water of differing temperatures and measure their subsequent heart rates.
Hypothesis
I think that the heart rate of the daphnia will increase up until around 40ËšC at which point most of the daphnia's enzymes will have denatured and rate of metabolism will have stopped or decreased sufficiently to have stopped the daphnia's heart rate.
Apparatus list
Test tube
Beaker
Different temperatures of water
Daphnia
Pipette
Stop clock
Microscope
Slide
Safety
Follow usual lab safety rules. There are no major safety considerations in this experiment as the daphnia aren't harmful and water above 40ËšC will not be used.
Method
To determine if temperature does have an effect on the daphnia, I intend to carry out the following experiment. The experiment will involve measuring the cardiac activity of the daphnia at different temperatures, ranging from very cold (approx. 5 degrees Celsius) to quite warm (approx. 40 degrees Celsius).
· A selection of similar sized/age Daphnia will be taken.
· A variety of different temperatures of water will be set up, these temperatures will be kept constant whilst the daphnia are submersed in them. The temperatures will be set up by using ice to cool tap water down to lower temperatures and boiling water to heat it up. The temperature will be measured to within 0.1°C on a mercury thermometer to ensure accuracy. The temperatures will range from 10°C to approximately 40°C and it will be done at 5°C intervals.
· The daphnia will be submersed in the water and left to equilibrate for 3 minutes, after this time one of the Daphnia will be removed and put onto a microscope slide, this will then be quickly put under the microscope. -1-
· Under the microscope, the Daphnia will be observed for 20 seconds, this heart rate will be counted and recorded, this can the be multiplied by 3 to give a beats per minute. This way, the beats counted will be at as close temperature as possible, as they won't have time to cool / warm significantly.
· The experiment will be repeated twice for each temperature.
Dependant variable:
It is important to ensure that all variables are kept constant, allowing the experiment to be a fair one, to do this the following precautions should be taken:
· The Daphnia used should be of a similar size and age to ensure that they react in the same way to the different temperatures.
· The heartbeat should be counted as soon as possible after the
Daphnia is placed under the microscope to ensure that the temperature it has equilibrated to remains as close as possible and is not affected by the light source of the microscope.
· All of the Daphnia should be allowed the same amount of time to equilibrate to the temperatures, thus they all experience the same conditions and this makes the experiment more accurate.
· The same person should always count the heartbeats to ensure that judgement errors do not affect the overall results.
· Each daphnia should have the same transfer time from water to microscope. (If time exceeds 15 seconds, void that part of experiment and repeat.)
· The temperature of the water needs to be monitored constantly to ensure that the results are being recorded at consistent temperatures.
Independent variable
· The temperature of the daphnia will be changed from 10˚C to 40˚C with 5˚C increments. This will be done by allowing the daphnia to acclimatise to different temperatures by putting their container in different temperatures of water. The results for experiment will each be repeated three times.
· The heart rate of the daphnia will be counted by looking at them under a microscope with 100x magnification and taking heart beat for
20 seconds and multiplying this figure by 3 to give beats per
Show More

Related Documents: daphnia heart rate Essay example

Daphnia Magna Heart Rate Lab Report

The effect of trisodium phosphate on Daphnia magna’s heart rate Abstract: Trisodium phosphate is a key regulator of body systems such as the heart. The heart rate of Daphnia magna can be influenced by many factors, since these water dwelling creatures are quite small. To find the change in heartbeat of the D. magna, we counted the heart beats of D. magna that were soaked in either water or trisodium phosphate. There was a significant difference between the heartbeat of the D. magna in the different…

Words 1782 - Pages 8

Daphnia Experiment

Factors Affecting Heart Rate Studying How Different Environments Affect the Heart Rate of Daphnia Emily Wright (0522016): Year 11 Biology Tania Balind  Table of Contents Factors Affecting Heart Rate 1 Abstract 2 Introduction 2 Method 3 Risk Assessment and Ethical Considerations 4 Results 4 Discussion 5 Appendix 6 Abstract This experiment aimed to find the effects of different substances on heart rate in Daphnia Magna. The two substances used…

Words 1575 - Pages 7

Daphnia Lab Report

Discussion on the effect of alcohol on Daphnia Magan The experiment on the effect of alcohol on the heart rate of Daphnia was done in Richland college lab. The purpose was to see how 1% , 3%, and 5% of alcohol concentration affects the heart rate of the Daphnia per minute. The hypotese being tested in this investigation is, The increase of alcohol in bloodstream decreases the heart rate per minute. The prediction was that the Daphnia heart will decrease and will eventually stop when it is exposed…

Words 406 - Pages 2

Daphnia Experiment

results would suggest that each of the solutions besides the control that the Daphnia were bathed in had an effect on how the neurons on the heart of the Daphnia produced rhythmic outputs to the rest of its body. This indicates that external factors have an effect on the heart rate of Daphnia. For example, in the control or room temperature test, the heart rate for that Daphnia was a normal rate, but at 5℃ the heart rate decreased a lot. The results for the control and the 5℃ water were as expected…

Words 1076 - Pages 5

Plant Defenses (Daphnia) Experiment

Defenses (Daphnia) Experiment Author’s Name Professors Name Institutional Affiliation Date Abstract The experiment was carried out to determine the effect of plant defenses on Daphnia magna which is a small herbivore. The plant defense that was used in this experiment was black and green tea. The Daphnia Magna was placed in on a microscope and the HR (heart rate) per minute was recorded. After the introduction of black and green tea to the small herbivore, the heart rate was recorded…

Words 1661 - Pages 7

Daphnia Magna Experiment

on the heartbeat rate of Daphnia magna was studied using variations of temperature. To determine whether the Daphnia species heartbeat rate is influenced by temperature, the effects of three different temperatures (±5oC, ±16oC, ±34oC) was investigated. Daphnia magna is a crustacean, that lives in aquatic environments. Predictions were based on the knowledge that physiological processes increase at higher temperatures, therefore, it was hypothesized that the Daphnia's heart rate will be faster at…

Words 881 - Pages 4

The effect of caffeine on the heart rat Essay

The effect of caffeine on the heart rate of Daphnia AIM: In this experiment I am going to investigate the effect of different concentrations of caffeine on the heart rate of Daphnia. My hypothesis is that the higher the concentration of caffeine, the higher the heart rate. Also, I think that there would be a concentration of caffeine where the daphnia would not be able to live. INTRODUCTION: Daphnia are tiny crustaceans which are about 0.2 to 5 mm that live mainly in fresh water; lakes, rivers…

Words 1279 - Pages 6

Daphine Heart Rate Lab Report

one daphnia, from the daphnia jar to a clean depression slide, which was then transferred onto a microscope. The microscope was focused on the daphnia so that the heart is in view, and then a researcher recorded the number of heart beats for a 10 second interval. This first observation determined the base heart rate of the first daphnia. Daphnia were then removed from the microscope and one drop of the test solution was added to the daphnia. A ten-minute time period was allotted for daphnia HR to…

Words 502 - Pages 3

Daphnia Research Paper

Introduction Daphnia or "water fleas," are small aquatic crustaceans. They live in a variety of aquatic habitats, including freshwater lakes, streams, ponds, and rivers. These organisms are useful for biological research due to their important interactions within their ecological niche. An ecological niche is the role and position that a species occupies within its ecosystem. Daphnia has a myogenic heart which differs in structure from human hearts but shares similarities in the way it is controlled…

Words 2237 - Pages 9

Daphnia Magna Lab Report

INVERTEBRATE RESPONSES TO STIMULI NAME : SHARAVANI SHANMUGANATHAN SANJEEVI COURSE : BIOLOGY (SBI4U) INSTRUCTOR : MS. PAULINE COLE INSTITUTION : SUNWAY COLLEGE DATE : 3 MAY 2016 Introduction A physical description about the Daphnia Magna would be that these creatures has a body, shaped like a kidney bean, approximately 2 – 5 mm in size and being transparent. The body colour that this organism reflects is basically the colour of the food consumed by it. The transparent body is covered by a shell…

Words 2161 - Pages 9