Essay on Dna Extraction

Submitted By NigeranSpeed4
Words: 889
Pages: 4
Open Document
Pre IB Biology Lab #3: DNA Extraction

Prepared For: Courtney Oliver By: Adefemi Obisanya Date: December 21, 2012 In this lab, the researcher purpose for conducting this experiment is to become familiar with a procedure for extracting DNA, collect DNA samples and observe the physical characteristics of DNA. The hypothesis is that the DNA extracted from the strawberry will look like clump strands that cannot be seen by the naked eye. Materials
Zip lock sandwich bag
1 ripe strawberry
Lysis buffer
Coffee filter
Erlenmeyer flask
Graduated cylinder
Small funnel
Glass slide
Ethyl or isopropyl alcohol
Ice water bath
Test tube
Plastic stirrer
Scissors
Safety goggles
Procedure
1. Obtain 1 fresh strawberry. Remove any of the leaves if they are attached.
2. Place the strawberry in the zip lock bag. Squeeze all the air out and seal the bag tight.
3. Gently mash the strawberry in the bag with your fingers for at least 5 minutes. Do not break the bag
4. At the end of 5 minutes, add 10 m-ml of the lysis buffer to the bag. Squeeze the air out again and seal the bag tight.
5. Mash the bag with the lysis buffer for 2 minutes. CAUTION: Mash the mixture carefully; the fewer bubbles created the better the results.
6. Place the funnel in the flask and place the coffee filter in the funnel.
7. Cut one of the bottom corners of the baggie off and slowly squeeze the strawberry liquid and the pulp into the funnel.
8. Allow the liquid to flow into the flask. Do not force the liquid.
9. Pour the filtrate into a clean test tube making sure to leave room for the alcohol
10. Slowly pour ice cold alcohol down the side of the test tube so it forms a separate layer on top of the strawberry liquid. Do not pour the alcohol directly into the filtrate.
11. Wait about a minute and observe the formation of a thin cloudy layer in between the two liquids. This is DNA.
12. Take the plastic stirrer and gently dip the tip into the DNA fibers and slowly pull the fibers up and place a small sample on a clean glass slide

Of the data collected, it can be said that the DNA was shown and extracted in the experiment and the DNA looked like small microscopic bubbles and had a resemblance that reminded me of lint. After the mashing, the bubbles appear in multitudes, but subsided in later part.
Assigned Questions:
1. Define: Diploid, octoploidy and filtrate.
A. Diploid - A cell or an organism consisting of two sets of chromosomes: usually, one set from the mother and another set from the father. In a diploid state the haploid number is doubled, thus, this condition is also known as 2n.
B. Octoploidy - having a chromosome number eight times the basic haploid chromosome number.
C. Filtrate - To put or go through a filter
2. Describe the function of DNA molecule be found
DNA is the blueprint that allows a plant or animal to divide itself, from a single-cell, into an extremely complex organism with many different kinds of cells and containing many millions of cells. DNA also allows for both sexual and asexual reproduction, depending on the organism in question. It also takes noting that DNA is what is responsible for the variety of plants and animals that exist due to its central role in evolution. Small mutations of DNA information, over long periods of time, lead to
Show More

Related Documents: Essay on Dna Extraction

Fruit DNA Extraction Copy Essay

Fruit DNA Extraction Introduction: All living things come with a set of instructions stored in their deoxyribonucleic acid (DNA), that determine the way you look and how your body functions. Whether you are a human, cat, strawberry, or bacteria, each cell has DNA contained in it (with some extremely rare exceptions), and is located in the cell nucleus. DNA contains the instructions needed for an organism to develop, survive and reproduce. It is the blueprint for everything that happens inside…

Words 1055 - Pages 5

Straw Berry Extraction Essay

1/eb Extraction of strawberry DNA Background Research Since DNA is the blueprint for life, everything living contains DNA. DNA isolation is one of the most basic and essential techniques in the study of DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders, produce DNA fingerprints…

Words 509 - Pages 3

Essay on BLA BLA BLA

Strawberry DNA Extraction Lab by C. Kohn, WUHS and Stacy Fritz, NAAE Name: Hour Date: Date Assignment is due: At end of hour Why late? Score: /10 Day of Week Date If your project was late, describe why Materials measuring cup measuring spoons ice-cold rubbing alcohol 1/2 teaspoon salt 1/3 cup water 1 tbsp Dawn dishwashing detergent cheesecloth Re-closable plastic sandwich bags test tube (or small glass jar) 3 strawberries (green…

Words 410 - Pages 2

Dna Synthesis Lab Report

Introduction: DNA extraction, PCR, and gel electrophoresis were used to determine the sex of a diecious plant: Silene latifolia. Unlike most plants that are hermaphroditic, diecious plants are either male (XY) or female (XX). The presence of the Y chromosome in a male diecious plant presented a point in which the DNA was be isolated, amplified, and used to differentiate between plant sexes. To identify the sexes three techniques were divided among a couple of weeks. The first was DNA extraction. DNA extraction…

Words 1206 - Pages 5

Electrophoretic Assay Lab Report

due to current techniques for tissue sampling and statistical analysis (Avise, Nelson & Prodohl 1996). Application of molecular genetic assays was utilized to identify a sample of tissue with an unknown identity. Initially, deoxyribonucleic acid (DNA) was extracted and assayed in an electrophoretic gel for necessary content (Campbell, Harriss, Elphinstone, & Baverstock 1995).…

Words 2320 - Pages 10

DNA Science Lab: Review Test Essay

DNA Extraction: Genomic DNA is found in the nucleus of eukaryotic cells. Mitochondria contain single­stranded DNA from our mother. Reagents Used in Lab: ● Lysis Buffer: Disolves the cells’ nuclear membranes and the phospholipid bilayer, allowing for the DNA to be released ● Protease: Breaks down cellular proteins. We used protease to DESTROY DNAses and the histones. ● Cold Alcohol: to precipitate the DNA out of the solution. The DNA is less soluble in alcohol, so it WILL become visible…

Words 462 - Pages 2

plant photosynthesis Essay

Lab Exercise DNA Extraction 10/30/13 DNA in all organisms is found primarily in chromosomes. Eukaryotic cells, like those found in humans, contain several linear chromosomes. Prokaryotic cells, such as bacteria, contain one large circular chromosome. DNA is composed of building blocks called nucleotides, each made up of a five-carbon sugar (deoxyribose), a phosphate group, and a nitrogenous base. The four kinds of nucleotides differ only in their nitrogenous bases. It is…

Words 825 - Pages 4

Genetics Module 7 Lab 2 Essay

******************************************************************************************** Answer Sheet—Module 7 Lab DNA Extraction Click on the following link and view the DNA extraction: http://learn.genetics.utah.edu/content/labs/extraction/ 1. What is the source of the cells used in this demonstration? A human. 2. Give three practical uses of DNA that is extracted: a. Genetic testing b. Body Identification c. Forensic analysis 3. Name the piece of equipment…

Words 1689 - Pages 7

Analysis MtDNA Essay

Mitochondrial DNA Lab CONCEPTUAL OVERVIEW In this lab, you will isolate, amplify, and sequence a piece of DNA from the mitochondria of your own cheek cells. The lab will introduce you to several vital biological concepts and techniques: • Mitochondrial DNA. The fact that mitochondrial DNA have their own DNA is powerful evidence that they originated from the engulfment of prokaryotic cells by other cells. A question to think about: in this lab, how will you distinguish between your…

Words 5017 - Pages 21

Dna Testing Lab Report

Introduction DNA analysis is the process in which evaluates the genetic material so that it can be used to identify individuals for use in a forensic/criminal investigation. DNA, or deoxyribonucleic acid, is the hereditary material that is found in almost all organisms. It is found in the nucleus of the cell and also small amounts can be found in the mitochondria and nearly each cell in a human’s body has the same DNA. But what is DNA made up of? Well it is made up of a code of four chemical bases…

Words 831 - Pages 4