In this Lab experiment, how fermentation and cellular respiration change with different carbohydrates. Cellular respiration is the process that is done by many organisms to convert organic molecules into energy. Fermentation is different in the fact that it is done without O2. The equation for fermentation is C6H12O6⇒2C2H5OH+2ATP. This fact makes fermentation an anaerobic function, because it doesn't use Oxygen. Cellular respiration is an aerobic function, because oxygen is necessary for it to be carried out. many bacteria can use fermentation, and for it to happen, there has to be no oxygen, and warmer temperatures speed up the reaction. The type of fermentation in this lab is called alcohol fermentation, and yeast is used to measure how much CO2 is produced from this form of fermentation when different sugars are added. The reason for this lab is to better understand why and how this bacteria can use this form of cellular respiration. To test the different conditions that yeast might be in, and to see there effects, multiple test tubes with yeast in them will be exposed to different carbohydrates like Glucose, Maltose, and Starch. There also will be a control which will just have water added. Also tested will be temperature. one of each of the yeast mixtures will be put into either a warm bath or kept at room temperature. Before the experiments it is believed that the yeast with starch added, and kept in the warm bath will perform the best. For this lab, few materials will be needed, including 8 large test tubes, 8 small test tubes, a wax pencil, 250ml beaker, graduated cylinder, yeast, Glucose, Maltose, Starch. now the first step is to label the large and small test tubes 1-6, and measure how much liquid can be held in the small test tube, and record this number. next fill the small test tubes two-thirds of the way full with the yeast solution, each tube should have the same volume. next fill the last third of the test tubes with the carbohydrates. test tubes 1-2 will have Glucose, 3-4 will have Maltose, 5-6 will have starch, and 7-8 will have water. This step is important, because these test tubes must be filled to the absolute top to avoid any air from getting in. Now over a sink, put the large test tubes over the small ones, and push the small test tubes to the very top. Then invert both tubes put together
