Forensic Dna Lab Report

Words: 852
Pages: 4
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I. Thesis

Criminal investigators with the help of forensic DNA can identify possible perpetrator.

II. Introduction
The principle of this experiment is to have a thorough perceptive of forensics and how it used to aid in the criminal justice system. This experiment will examine the potential for live bacteria in eight different locations in the household. In addition, this lab will utilize the Fomite Transmission and show how live bacteria left at a crime scene can assist law enforcement in the capture and prosecution of a criminal. The prediction for this research, is that out of the eight surfaces being tested, the bottom of the shoe will have the most …show more content…
Procedure

Exercise 1: Fomite Transmission
Part 1 of 2

1) For this experiment items needed are: Two prepared agar plates, bleach, camera, two disposable cups, hand soap, Isopropyl alcohol, permanent marker, paper towels, a pair of gloves, apron, safety goggles, face mask, eight sterile swabs and distilled water.
2) Disinfect the work area with bleach and paper towels. Then, wash hands with soap and water, put on the apron, as well as, the safety goggles, face mask and gloves.
3) Once agar plate is prepared and cooled, label the plate on the backside using the permanent marker, divided into four quadrants on the backside of each plate. On the first plate label each quadrant one through four and the second plate label five through eight. Fill one of the disposable cups ¼ with distilled water and the other disposable cup ¼ with bleach. Label the disposable cups on the outside with the permanent marker what is contained inside the cups.
4) Next, place eight swabs in the disposable cup of distilled water. The eight areas that will be swabbed for this experiment will be: 1. Toilet Handle
2. Door Handle
3. Sink Handle
4. Stairway Railing
5. Bottom of a Sneaker
6. Computer Keyboard
7. Cell …show more content…
4
Stairway Railing
8 Colonies, vary from small to large, circular, flat, yellowish white in color.
5
Toilet Handle
1 Colony, very large, irregular, lobate, Crateriform, dull white in color.
6
Computer Keyboard
6 Colonies, small pinpoints, circular shaped, raised, shiny, opaque white in color.
7
Cell Phone
1 Colony, very large, irregular, Crateriform, dull, opaque white in color.
8
Kitchen Light Switch
3 Colonies (2 pin point/1 medium), Umbonate, shiny, opaque white in color.

V. Conclusion

The conclusion of this experiment is to detect fomite transmission in the household and how they aid in forensic science. Eight surfaces were tested in various locations and incubated for 48 hours. Inspection of the eight segmented areas of the inoculated agar plate, the bottom of the shoe had the most overall bacteria growth, containing 16 colonies. The following is a list of the incubated plate areas in descending order of overall growth:
* Bottom of a Shoe 16 Colonies
* Door Handle (Hallway) 5
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