Genomic variation of Georgetown local populations of Fundulus heteroclitus using microsatellite loci
Maritza Espinoza and Crisfer Fernandez-Reyes
How to make Tepper kick his C.lupus by using an experiment with F.heteroclitus and microsatellites
Introduction: Studying the effects of environment and its variation in an organism’s genome is important in understanding the concept of genetics. While generally much of genetics relies in the understanding of DNA and RNA, environmental factors play a large role in phenotypic expression which correlates to minor changes in the genotype. One of the ways to understand this is by the use of the mummichog. The Fundulus heteroclitus (mummichog), is a small killifish found in estuarine water systems (area where a river flows into the sea) such as salt marshes/ponds or brackish waters typically found in the Eastern/Atlantic coast of the United…show more content… heteroclitus fin clips collected from the four different regions of the Winyah Bay. Through a partnership involving the University of South Carolina, Cornell obtained fin clip samples of mummichogs collected from the Winyah Bay Estuarine system in Georgetown, South Carolina. The DNA was isolated using the DNeasy Blood and Tissue Kit (Qiagen). The fin clips were digested with buffer ATL and Proteinase K by incubating at 56ºC for 2 hrs. The DNA was ethanol precipitated with buffer AL. The DNA was centrifuged at 8,000 rpm for 1 minute and washed with buffer AW1. The centrifugation was repeated. The samples were washed with buffer AW2 and centrifuged for 14,000 rpm for 3 minutes. The DNA was eluted from the column with buffer AE, incubated at room temperature for 1 minute, and centrifuged at 8,000 rpm for 1 minute. The column was washed with buffer AE and the process was repeated. DNA mass was determined by scanning the DNA on the Nanodrop Spectrophotometer from 260-230 nm with peak absorbance values for “pure” nucleic acids averaging at a ratio of
