How Does Dnase Work

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1. How does DNase work? Is the enzyme used site specific for cleavage? Why doesn’t DNase work on RNA?
- What DNase does is denature in DNA proteins and leaves any RNA intact. It cleaves specifically at DNase hypersensitive sites. DNase doesn’t work on RNA because DNA and RNA are two different things and DNase will not denature RNA due to it having different properties.
2. Why is it important to remove DNA contamination?
- It is important to remove any DNA contamination to make sure you don’t have any false amplification of your sample. Any DNA that is left will give you false amplification.
3. How could genomic DNA presence interfere with cDNA synthesis using RNA template?
- It may yield wrong results during the amplification step and amplify
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