LB liquid medium (pH = 7.0-7.2): yeast extract 5 g, tryptone 10 g, sodium chloride 10 g, double distilled water set to 1 L then autoclaved for 30 min and stored in the 4 ° C.
LB solid medium: agar powder was added to LB liquid medium to a final concentration of 1.7% and autoclaved for 30 min.
DAP stock solution (50 mg / mL): 1 g of DAP was dissolved in 20 mL of sterile water and sterilized by a 0.22 μm filter. Storage was performed at -20 ° C and the working concentration was adjusted at 50 μg / mL.
SOB liquid medium: tryptone 20g, yeast extract 5g , NaCl 0.5g, 250 mol/L KCl 10ml solution, add distilled water 1L and then adjusted the solution pH by 5 mol/L NaOH (pH = 7). Then after, add water to 1L volume in the 15psi and steam sterilize for 20 min under high-pressure. The solution was added into the 2 mol/L, MgCl2 solution 5ml and sterilized before use.…show more content… Phosphate buffer saline (PBS): 8 g NaCl, 2.9 g Na2HPO4·12H2O, 0.2 g KH2PO4 and 0.2 g KCl diluted and then sterilized with deionized water to 1000 mL, and
