This process is driven by a physical interaction between myosin heads and adjacent actin filaments, which is governed by the regulatory proteins troponin and tropomyosin. Troponin is a Ca++ binding protein that allows myosin to bind by controlling the shape of tropomyosin, which is part of the thin filament actin. When action potentials have been sent from the central nervous system to the effector neurons, muscles receive the signals through T-tubules of the sarcolemma which then triggers release of Ca++. The Ca++ ions then bind to troponin which moves tropomyosin from its blocking position and allows myosin to bind to actin (Johnsen, p. 38). Subsequent binding and hydrolysis of ATP to the myosin head helps to produce the conformational change to the myosin head in order to produce the power strokes that move actin filaments.
In this lab exercise, we studied the responses of many muscle fibers with their motor neurons attached, specifically, a frog gastrocnemius. The property of excitable tissue is defined by the all-or-nothing law where the nature of the response does not depend on the strength of the stimulus, as long as the strength reached threshold. What we were interested in studying however, was how the strength of the muscle response (or tension developed) can be