Natural Rubber Latex Case Study

Words: 1950

Pages: 8

Open Document

Natural rubber latex is one of the problems that raises the environmental concerns. In this study the degrading ability of Ficus elastica rubber latex by a bacterium strain ASU-03, isolated from Egyptian soil was assessed. The strain was able to produce clear zone around its colony on latex rubber containing medium and was identified by conventional methods as Streptomyces sp. Phylogenetic analysis of 16S rRNA (16S rRNA) and RNA polymerase β-subunit (rpoB) genes were applied. Results of the 16S rRNA gene analysis revealed that the strain was highly related to Streptomyces spp. (100% similarity), so the rpoB gene was partially sequenced to clarify the specific name of the isolate. Phylogenetic tree based on rpoB gene sequences indicated that …show more content…
10 ml of fuchsin reagent was added to the samples (50 μl of the NR dissolved in n-hexane) as described by [ 16 ]. The composition of the fuchsin reagent was prepared as follow: 2 g of fuchsin dissolved in 50 ml of glacial acetic acid plus 10 g of Na2S2O5 plus 100 ml of 0.1 N HCl + 50 ml of H2O [ 17 ]. Positive results are indicated by purple color at room temperature after 30 min. Scanning electron microscopy (SEM)
The morphological change during growth of the bacteria on NR was also assessed by SEM. The inoculated rubber samples as well as the control were fixed overnight in 5% glutaraldehyde, and then dried at 50 ºC. The samples were mounted on metal stubs and coated with gold and palladium (Jeol JFC1100E Iosputtering Device). Micrographs were taken by means of a Joel JSM-4500 LV electron microscope operating at 15 KV in Electron Microscopy Unit, Assiut University.
Phenotypic and molecular identification of the bacterial strain Phenotypic identification
The isolate was identified based on its morphological and biochemical properties using Bergey’s Manual of Systematic Bacteriology [

Pros And Cons Of Birth Control

Birth control would be provided by free clinics who would reinforce teaching of birth control and its effects. Birth control first became a contraceptive and a control on fertility. Over the years there have been many different methods and in some cases devices that were used to prevent pregnancy. At one point, abortion was even considered a form of birth control.…

Words 1042 - Pages 5

Agriculture and Plants Essay

Title: Hartmann's Plant Science: Growth, Development, & Utilization 3rd ed. Material in a format for use by print disabled student only. Further reproduction or distribution of this material is an infringement of copyright law. Chapter 1 Plants: Structure, Classification, The Role of Cultivated Plants in the Living World After reading the chapter you should be able to: Understand the role of cultivated plants as food sources. Understand the benefits of cultivated plants in…

Words 4917 - Pages 20

Fixed Cost and Ms Sandra Lazzarini Essay

Harvard Business School 9-187-098 Rev. February 15, 2000 Polysar Limited As soon as Pierre Choquette received the September Report of Operations for NASA Rubber (Exhibits 1 and 2), he called Alf Devereux, Controller, and Ron Britton, Sales Manager, into his office to discuss the year-to-date results. Next week, he would make his presentation to the Board of Directors and the results for his division for the first nine months of the year were not as good as expected. Pierre knew that the…

Words 4085 - Pages 17

Contraception And Abortion

higher than normal levels for 21 days then no pill for 7 days, and then repeat Some are for longer time frames ie. Seasonale; 84 days of combined hormones- aka only have 1 period every three months How it works Mainly by preventing ovulation In natural cycle, low levels of estrogen during and after menstrual period trigger the pituitary to produce FSH, which then stimulates the ovulation process Estrogen levels are made high which inhibits the production and so the signal to start ovulation never…

Words 3038 - Pages 13

Essay about Marketing Principles Assignment

discus the macro environmental forces and key trends in personal computer and snack foods industries. Macro environment consists of six larger societal factors that affect the micro environment. The forces are demographic environment, economic, natural environment, technological environment, political environment and cultural environment. In part two of the assignment has three research situations. Each of the situations will be discussed that what type of data collection method is suitable…

Words 3949 - Pages 16

Sexually Transmitted Diseases Essay

100-113 Soils 114-128 Unit 6 Living things in the Environment Plant and animal cells 129 Eukaryotes vs prokaryotes 130 Structures and functions 133-137 Microbes 148-150 Onion and cheek cells 150-151 Ecological study 154 Seed…

Words 35677 - Pages 143

Health, Safety and Welfare in Ecce Setting Essay

Table of contents: Aim and Introduction. Health and safety in ECCE setting. Health and Safety Policies and Procedures in ECCE Setting. Well-being of children in ECCE setting. Characteristic of positive environment in ECCE setting The factors that contribute to the overall well-being of the children in ECCE setting Components of a balanced diet for children Menu sample. Indoor and Outdoor Safety. Signs and symptoms of unwell child. Crèche Safety Check list Sample…

Words 19654 - Pages 79