Ninhydrin, biuret, orcinol, I2KI, and Nile blue are all the agents that can be used to detect and measure specific classes of biochemical. Ninhydrin was used to test for amino acids. When a sample containing amino acids is mixed with ninhydrin and heated to 80℃ the color produced with be blue. Biuret was used to test for proteins. When a sample containing proteins is mixed with the biuret reagent and heated to 25℃ the color produced with be purple. Orcinol was used to test for sugars. When a sample containing sugars is mixed with orcinol and heated to 80℃ the color produced with be blue-green. I2KI was used to test for starch. When a sample containing starch is mixed with I2KI and heated to 25℃ the color produced with be blue. Nile blue was
