In order to verify different aspects of our capstone project, we will be building three different prototype. Our first and main prototype is based off of the gold disk electrode system. We will be using the gold disk electrode as a working electrode, a platinum wire as the counter electrode and a silver wire as the reference electrode. A ferricyanide solution will act as our redox probe. An alternating current and voltage are then input into this electrochemical cell and the output impedances values can then be used to characterize the different markers. In order to properly characterize each biomarker we will first begin by cleaning and sonicating the device. Cyclic voltammetry and AC impedance are then conducted to figure out the formal potential…show more content… These activated carboxylic acid groups can then bind to the amine groups on the antibody. Ethanolamine then acts a blocking agent and allows the antigen to bind to the antibody properly. Depending on the antigen- antibody pair they will all have different optimal binding frequencies. These optimal binding frequencies must first be characterized for each biomarker and the dynamic frequency range can then be used to check for the presence of the different markers. We will be conducting different design of experiments on this prototype to check for different factors. The prototype will be used to check for different specifications such as multimarker detection, faster detection times, reliability of the sensor, validity of the results, smaller sample volume. The data obtained from the electrochemical impedance spectroscopy will also be compared to the current gold standard method - ELISA. In order to check if the sensor can detect multiple markers we will be utilizing two already characterized markers (such as Insulin) and check to see if the sensor can detect both
