Nt1310 Unit 3 Lab Report

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LABORATORY EXERCISE QUESTIONS

~~1. What type of microscope would be used in the laboratory to examine the cell size and arrangement within a tissue sample? Please give reasoning behind the answer. Which microscope would be useful when studying the internal structures of a single-celled organism? (2 points) I would use the compound light microscope to examine the cell size and arrangement as the compound light microscope is up to 1000x. This microscope will show the cell size and organization of the tissue sample. To study the internal structure of a single- celled organism I would use the transmission electron microscope.
~~2. List two careers where microscopes are used. Describe in what capacity. (2 points)
• Microbiologists- This career …show more content…
Diaphragm
h. Coarse focusing knob
i. Illuminator
j. Base

~~5. What type of microscope has primarily been used in this lab? (1 point) The compound light microscope was used in this lab.
~~6. State the function of the following parts of the microscope: (3 points)
A. mechanical stage- This is the area where a specimen is placed on the microscope for viewing.
B. ocular lens- This is used to magnify the image that has been formed by the objective lens.
C. coarse and fine focus knobs- The knobs bring the specimen into focus by lowering the stage or by raising and lowering the upper body tube.
~~7. State how to adjust each of the following components of the microscope (2 points)
A. Diopter- To adjust the Diopter, I would adjustment the focus by viewing with my right eye in the fixed ocular, and then using my left eye I will move the serrated diopter right to adjust the focus for the left eye.
B. interpupillary distance-The scales are used by moving the oculars together or apart.
~~8. What is the total magnification when using a compound light microscope with an objective lens of 100X and an ocular lens of 10X? Show the calculation used to derive the answer. (2 …show more content…
Why are coverslips used to observe specimens under the microscope? (1 point)
The purpose of using a coverslip is to hold the specimen in place while also protecting the objective lens from contamination.

~~10. Letter "e" exercise:
A. Describe how the orientation of a letter "e" changes when it is viewed under the microscope. (2 points) The letter e in the experiment is viewed upside down and backwards.
B. If the slide is moved to the right on the mechanical stage, in what direction does the specimen appear to move while observing it under the microscope? (1 point) If the slide is moved to the right, the specimen moves to the left during observation.
~~11. If the ocular magnification is 10X, under what total magnification was the sequence of colors most clearly seen from the colored threads exercise? Propose a reason why this might be. (2 points)100x, and the reason it was the best one was due to the clarity.

~~12. What differences are observed in the various tissues/organelles between the stained, prepared slides found in Procedures #4 through #8? (2 points) The cells differed on both slides because the plant epidermis cell structure seemed to appear close together, and the spirogyra was spread
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