Pglo Transformation Lab Report Essay

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Transformation Lab Report

Introduction
Transformation is the transfers of virulence from one cell to another, through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith, a British microbiologist. Griffith observed that the mutant form, non-virulent form, of the bacteria Streptococcus Pnumoniae could be transformed into the normal, virulent form, when injected into mice along with heat killed normal forms. He concluded that somehow the information the dead virulent form had transformed the mutant form into a virulent form.

Later on through the works of Avery, Macleod, and McCarty in 1944, it became obvious that DNA is the transforming property and the substance …show more content…
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| |LB/AMP/ARA (+) |LB/AMP (+) |LB/AMP (-) |LB (-) |
|Type of Reaction |COLONY |COLONY |NO REACTION |LAWN |
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+ Pglo Transformation Lab Report

process of transformation was green fluorescent protein. Two tubes were labeled “+pGlo” and “-pGlo” and 250 μl of CaCl was added and tubes were placed on ice. I used a sterile loop and extracted 3 large circular colonies of E. coli then swirled it into the +pGlo tube and repeated this process for –pGlo tube. Under UV light both tubes displayed no color difference. The tubes were placed on ice for 10 minutes then heat shocked at 42 ˚C for exactly 50 seconds in order for transformation to occur with…

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Pglo Transformation Lab Report

Coli Bacteria with the Gene GFP (green fluorescent protein) By Using pGLO As a Vector Introduction: Genetic transformation is the alteration of a cell by taking DNA from another organism and expressing those new characteristics from the organism of which it took the DNA from (Julie Carnagie and Leonard C. Bruno 2006). The cells that are able to take up DNA from other organisms are known as competent. This genetic transformation is used for many different things, from making medicines to DNA cloning…

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Pglo Transformation Lab Report

Purpose: This investigation is demonstrating the act of genetic transformation. We inserted a gene into the bacterium E. coli. Gene being a piece of DNA which provides the instructions for making a protein. The main chemical components used in this lab were calcium chloride, E. coli bacterium, a broth (made up of carbohydrates, amino acids, vitamins, nucleotides, and salts that allow bacteria to grow), pGLO plasmid (contains arabinose operon that turns on when the arabinose sugar is present, GFP…

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Pglo Transformation Essay

Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab, the Green Fluorescent Protein, which is typically found in the bioluminescent jellyfish Aequorea…

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Essay about Pglo Transformation Lab Final 1

pGLO Transformation Lab In this lab you will perform a procedure known as genetic transformation. This term means change caused by genes; it involves inserting a gene into an organism in order to change one or more characteristics in that organism. We will use E. coli K-12 as our host organism; ie the organism that will receive the gene. The gene we will transfer to E. coli K-12 codes for green fluorescent protein (GFP). The source of this gene is the bioluminescent jellyfish Aequorea Victoria…

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Lab Report Guidelines Essay

GUIDELINES FOR LAB REPORT Concise and Informative Title that Reflects The Findings of the Experiment [Arial font; 12 pt, bold, centered) A. Author and B. Author (MARB 301, Section 40x) [Arial font; 10 pt, bold, centered] Texas A&M University at Galveston, Genetics Lab (MARB 301), 200 Seawolf Parkway, Classroom Laboratory Building, Galveston, TX 77553 Summary Provide a brief summary of your findings in general terms that will entice me to read on  [Font: Arial, 10 pt*, left aligned] Up to 100 words…

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Essay The Antibiotic Resistance Problem

Staphylococcus and was on his way to remission. However, the Escherichia aureus (MRSA) infection while in the hospital, spendcoli infection responsible for his high fever was proving ing three months in the hospital fighting the infecintractable. Lab results revealed that it was resistant to eight tion. He survived, but lost 30 pounds and much of his different antibiotics, including the newer cephalosporins. strength in the battle. It took him almost two years to On Day 20 Mr. C lost consciousness…

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