Red 40 Dyes Lab Report

Words: 400
Pages: 2

Introduction
The Purpose of this STEM project was to do the quantitative analysis, meaning determining the amount of red 40 dye in each of the red M&M and red Skittle. Food dyes are very controversial and red dye is one of the most dangerous one of them, hence the determination of how much of that dye is present by the process of spectrophometric analysis.
Procedure
Firstly the candies were dissolved in water. Secondly, all remaining solid particles were filtered out. Then the samples were placed in the UV spectrophotometer to measure the absorbance of each sample at 504nm. Finally to generate a standardization curve eight standards were prepared using 50ml of stock solution provided by the chemistry stock room.
Results (Data)
Table 1: Absorbance
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Discussion
The results were obtained after trial and error. The cloudier the sample, the harder it is for the light to pass through it, so filtering our solution to the fullest was a very important factor. This was the key to perfection and the results are fantastic. Even though the results were obtained at a slow rate of speed, any small error could have been damaging. In my opinion this experiment was could have been more accurate. Which brings us to what exactly can we use to get even better results? Getting better quality candies, not old and dirty. Also vacuum filter technique was one of our biggest downfall in this whole experiment and some specially prepared filter beds would be a better solution.
Conclusion
Unfortunately, conclusion for this topic is not possible without the FDA, understanding the devastating effect of food dyes. There is no surprise that some of the other countries have banned these dyes. Although late, it is time for the FDA to realize their mistakes and ban these dyes from our