Red Food Dye Synthesis Lab Report

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Discussion and Scientific Explanations

The prepared solutions underwent a spectrophotometer analysis in order to determine absorbance and percent transmittance values for each solution. This data would provide the maximum wavelength, where the highest absorbance readings occur, in reference to each solution.

The highest absorbency for the solution containing red food dye occurred when the Spec 20 was set at 500nm. However, the largest percent transmittance occurred at 400nm. This data is supported in Graph 1 and Graph 2. The solution comprised of yellow food dye had a high absorbency reading at 400nm versus a large transmittance reading at 500nm. This data is presented in Graph 3 and Graph 4. The green dye solution generated a high absorbency …show more content…
It is important to note, however, that the color of light absorbed is not the same as the color of the solution. The color of a solution is only dependent on the wavelengths of light remaining once the rest of the light has been absorbed. For instance, green light usually ranges between 500 – 560nm. However, the green solution generated large percent transmittance values within the same wavelength range, Figure 6. This is attributed to the idea that a solution absorbs every frequency of light except for the range of light that is characteristic of the solution’s …show more content…
Before analyzing the phosphate concentration in each soda, the samples had to be degassed. Dissolved CO2, commonly referred to as carbonation, is characterized by a range absorbance values and percent transmittances that differ from PO4. Therefore, it was necessary to remove the dissolved CO2 from the soda samples as a means to prevent any discrepancies in the phosphate absorbency observations. Afterwards, the soda samples were diluted to a concentration 1/50 of their original concentrations. Ordinary soda contains phosphate levels too large for the Spec 20 to read. Therefore, appropriate concentrations needed to be prepared in order to garner adequate results. It is important to note that although dilutions occurred, the actual concentration of each soda remained unknown. Lastly, 5mL of AVM was added to each 10mL diluted, degassed soda sample. Once the samples of soda had been prepared, their relative absorbencies were observed with the Spec 20, set at the analytical wavelength from week two: 370nm. The absorbencies of Check soda, diet soda, and Sam’s soda were 0.28, 0.37, and 0.44 respectively, given in Figure 12. The absorbencies were substituted for y in the calibration curve, which was generated during week two. Accordingly, the phosphate concentrations or x values were calculated by the y substitution. The concentrations were then multiplied by a factor of 50 in order to determine each soda’s
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