IMMOBILISATION OF SACCHAROMYCES CEREVISIASE FOR THE PRODUCTION OF BIOETHANOL
The major focus of this paper is the different content of immobilizing yeast. The independent variable is calcium alginate and polyacrylamide gel. The hypothesis was not stated in this article; however the purpose of this experiment was to determine if calcium alginate or polyacrylamide gel would produce ethanol at a higher rate and also the productivity of ethanol. The topic of this article was on bioethanol, specifically on increasing production of ethanol. It will attempt to increase production using cell immobilization and in doing so with the use calcium alginate and polyacrylamide. The immobilization of yeast with polyacrylamide gel was produced by dissolving .16 grams of ammonium per sulfate, 0.5 mL of N,N,N’,N’-tetramethylethylenediamine, and 0.264g of N,N’-methylenebisacrylamide in 20 mL of distilled water. The solution then were put into different quantities of dry yeast and cooled to 0 degrees Celsius then nitrogen gas was purged in for 20 minutes soaking in thermostatic bath at -18 degrees Celsius for one day. The gels formed were cut into 5mm then washed out to clean out all soluble polyerms and unreacted monomers. The polyacrylamide will use different quantities of dry yeast 2, 4, 6, and 8 grams to create the gels. The calcium alginate was prepared by autoclaving sodium alginate and calcium chloride at 121 degrees Celsius for 15 minutes. The 100 grams of dry yeast was dissolved in 1L of distilled water and 2%, 3% and 4% of alginate was used to turn solution into
