The second reason is that America had developed the atomic bomb in June 1945. This played a major part in the change in Truman's attitude at the Potsdam Conference in the same year. The successful development of the atomic bomb had given Truman a lot of confidence, and this links back to his attitude that clashed with Stalin. Now Truman was even more arrogant and anti-communist, and as a result, it made Stalin even more determined to get his way.
The third reason is that as a result of Churchill's Iron Curtain Speech, the Truman Doctrine and Marshall Aid, Stalin had responded to these by forcing communism on Eastern Europe, then Comecon and Cominform, and hence the Berlin Crisis. This point marks the peak of the Cold War when relations between the USSR and the USA were at their worst. This was because of the Berlin Blockade, when America had humiliated Stalin, with Stalin on the verge between firing at the airplanes, or ignoring them and keeping the blockade up. This is another example of Stalin's stubborn nature, as seen in the Potsdam Conference mentioned in the paragraph two.
Finally, the fourth and last reason is