Sophorolipids are well known for their surface active potential in various industrial and household applications. They are utilized in laundry detergents (Hall et al. 1996), food industry (Akari and Akari 1987), cold storage transportation (Masaru et al. 2001), Bioremediation (Mulligan et al. 2001), cosmetic and dermatological products (Morya et al.2013), plant protection ((Kim et al. 2002), medical application as anticancer agent (Fu et al. (2008), anti-inflammatory or antiallergy agents (Hagler et al. 2007). Sophorolipids have recently emerged as promising molecules due to their broad-range functional properties and diverse synthetic capabilities of microorganisms with different carbon sources. [develter 2011, ashby 2006]
Sophorolipids are…show more content… Ltd, Mumbai, India and M/s S.D Fine Chemicals Ltd., Mumbai, India respectively. Sunflower oil used for production of sophorolipid procured from local vendor. Sunflower oil was analyzed for various tests as shown in Table 1 as per the AOCS official methods of analysis []
Microorganism
The sophorolipid producing strain Starmerella bombicola (ATCC 22214) was procured from Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Chandigarh, India as MTCC 1910. For antimicrobial testing E. coli, Bacillus subtilis and Propionibacterium acnes strains were obtained from the Microbial Type Culture Collection (MTCC), Chandigarh,…show more content… bombicola cells taken from the seed culture was added into a 250-mL Erlenmeyer flask containing 50 mL YEPD broth followed by incubation at 30⁰C 48 hr with shaking speed 210 rpm.at 210 rpm. This cell suspension was used as a inoculum (2%, v/v) in a sterile production medium (Zhou and Kosaric, 1995) with the following composition (g/L): yeast extract 4; urea 1; NaCl 0.1; MgSO4.7H2O 5; KH2PO4 1; FeCl3.6H2O 0.1; pH of the medium was adjusted to 4.5±0.1 using 1 N HCl (hydrochloric acid) before autoclaving. Two carbon sources were used in production medium. The primary carbon source was glucose and secondary carbon source used was sunflower oil (10%, w/v each). Glucose and mineral salt solution was sterilized separately by standard method of autoclaving at 121⁰C for 20 min and cool to room temperature before inoculation. The oil was autoclaved separately and added to the broth just before
