St Johns River Water Lab Report

Words: 1861
Pages: 8
Open Document
Quantification of Atrazine in St Johns River Water by HPLC-MS
NAME
EMAIL
12-1-17

Abstract
The use of synthetic chemicals in agricultural products has been shown to have undesirable consequences when these chemicals are released into aquatic environments at high levels. In order to determine the concentration of these chemicals and monitor them, methods must be developed of sufficient selectivity for single species in a complex mixture and sensitivity for trace concentrations. One such chemical is atrazine, known to cause endocrine related complications in many species and more. A method using solid phase extraction to concentrate atrazine from river water for quantification by high performance liquid chromatography tandem mass spectrometry …show more content…
One of the most commonly found chemicals in environmental samples is atrazine.1-3 Atrazine is an entirely man-made chemical, thus any presence in the environment is a residue from man-made products. While high enough levels of atrazine can be lethal, lower levels of atrazine are found most commonly and linger even after years with no additional exposure.3 The metabolites of degraded atrazine may be present at even higher levels and also persist for years.3 Both atrazine and its metabolites have been linked to numerous health issues across species from microorganisms to …show more content…
Figure 1 charts the standard curve and the linear regression equation and R2 value. Figure 1. A standard curve of the ratio of atrazine to the internal standard and the concentration of atrazine in ppb.
Four samples were extracted by SPE then analyzed by HPLC and MS in replicates of three. The peak area (AA) for the signal of atrazine and atrazine (D5) were determined. The averages of the three AA values for each sample analyzed were used to calculate the concentration of atrazine using the linear regression line equation. The mean AA values for each sample were used to create ratios of atrazine to the internal standard in order to calculate the concentrations were using the linear regression line equation. These values were recorded in Table 1 with their 95% confidence interval.
Table 1. A summary of the data collected by the HPLC-MS. The calculated mean ratio for each sample was used to calculate the mean concentration in ppb.
Sample Mean Atrazine (AA) Mean Atrazine D5 (AA) Mean Ratio Mean Concentration (ppb)
1 2 x 106 ± 3 x 106 5 x 106 ± 2 x 106 0.4 ± 0.3 3.4 ± 0.3
2 2 x 106 ± 9 x 105 6 x 106 ± 4 x 106 0.4 ±
Show More

Related Documents: St Johns River Water Lab Report

Essay on Finance: Decision Making and Willpower

Do You Suffer From Decision Fatigue? By JOHN TIERNEYThree men doing time in Israeli prisons recently appeared before a parole board consisting of a judge, a criminologist and a social worker. The three prisoners had completed at least two-thirds of their sentences, but the parole board granted freedom to only one of them. Guess which one: Case 1 (heard at 8:50 a.m.): An Arab Israeli serving a 30-month sentence for fraud. Case 2 (heard at 3:10 p.m.): A Jewish Israeli serving a 16-month sentence…

Words 5508 - Pages 23

Multiple Intelligences Seminar and Workshop Essay

Teaching and Learning through Multiple Intelligences Seminar/Workshop Content Outline: PART I – Explanation 1. What is the theory of multiple intelligences (M.I.)? 2. How does this theory differ from the traditional definition of intelligence? 3. What do multiple intelligences have to do with my classroom? 4. How has M.I. theory developed since it was introduced in 1983? 5. Who are the critics of this theory and what do they say? 6. What are some benefits of using the…

Words 14446 - Pages 58

Essay about An Introduction to Hydrophonics and Controlled Environment Agriculture

designs (including those with removable tops for Summer) to “force” bulbs and to grow flowers, citrus and other trees and shrubs in England, France, Germany, Sweden, The Netherlands, Spain and China. *1600’s – Heating systems “rediscovered”: steam/hot water, “bark” stoves (moist heat), manure as a heat source and charcoal heaters (though, high sulfur coal was found to be phytotoxic). Greenhouses were built with flues in the floor for below-ground heating. Note: Since greenhouses started in northern Europe…

Words 40128 - Pages 161

The Flaws Of Finance

GMO White Paper May 2012 The Flaws of Finance James Montier This paper is based on a speech delivered at the 65th Annual CFA Institute Conference in Chicago on May 6, 2012. A s a child, watching my parents write postcards whilst we were all on holiday was an instructive experience. My mother would meticulously write out the card, scattering a few interesting holiday tidbits within the text. My father, whose sum total of postcards sent was invariably just one (to his office), opted for a considerably…

Words 6387 - Pages 26

Managing Information Technology (7th Edition) Essay

from the Microsoft Corporation. This book is not sponsored or endorsed by or affiliated with the Microsoft Corporation. Copyright ©2012, 2009, 2005, 2002, 1999 Pearson Education, Inc., publishing as Prentice Hall, One Lake Street, Upper Saddle River, New Jersey 07458. All rights reserved. Manufactured in the United States of America. This publication is protected by Copyright, and permission should be obtained from the publisher prior to any prohibited reproduction, storage in a retrieval system…

Words 239873 - Pages 960