Midterm 1: study guide
Define:
Colony
Contaminant
Simple stain (direct and negative)
Answer:
Where do you dispose of the different types of waste in the lab?
What are the parts of the microscope and what are they for?
How does the oil immersion lens increase resolution?
What are the main ingredients of nutrient agar and what is the purpose of each?
What are the limitations of using petri plates for determining abundance and diversity of environmental samples?
What form of media is best for isolating bacteria? (broth, plate, slant?)
What are the steps of aseptic transfer of cultures?
What are the steps of smear preparation? What is the importance of heat fixing?
What is the difference between a basic and acidic stain (charge and what they stain)?
What are the steps of the gram stain, what does each reagent do (ex: crystal violet is the primary stain), and what do gram positive and negative cells look like after each step?
Can you identify gram positive and negative cells on a slide, with shape and arrangement?
What are common reasons why you may get an incorrect result with the gram stain?
What are the steps of the acid fast stain, what does each reagent do (ex: carbol fuchsin/phenol is the primary stain and mordant), and what color are acid fast and non-acid fast cells after the acid fast stain procedure? What makes a cell acid fast?
Can you identify samples with endospores, capsules, and