Tissue Banking Lab

Words: 1621
Pages: 7
Open Document
Introduction & Background
The first documented activity with tissues was in 1668, where a bone from a dog’s skull was used to repair defects in a human cranium. Ever since, the process of tissue banking has evolved and continues to be important to the health field by advancing many studies and improving modern medicine. Even though there are issues regarding the process of tissue banking, scientists are trying to come up with a standardized perfected method (Riegman et. al., 2008). During the process of finding a standardized method, our knowledge on different forms of cancer, transplants, and personalized medicine have increased. One specific problem that scientists are trying to improve is the problem with cell culture contamination and the …show more content…
(2014) prepared human, bacteria, and plant tissue samples (as shown in Table 1 below) to compare the differences in methods for preserving DNA in different storing temperatures. Similar to Riegman et al. (2008), Clermont (2014) did this to find a more efficient method for his procedures. This benefitted his research by creating an easier way to transport and store the samples. Different protocols were followed for each sample in order to extract the different types of DNA. The DNA from the bacteria was extracted using the Promega kit instructions, which was then dissolved in a rehydration solution. This solution was then stored at -20 degrees Celsius. To collect the DNA from the plant samples, 1 gram from the leaves of a grapevine was removed and then dissolved in a solution from the DNeasy Plant Maxi Kit. This was also stored at -20 degrees Celsius. The DNA of the human tissue samples was removed by a AutoGen machine to avoid the contact of chloroform. The red blood cells were broken down in buffer, while the white bloods cells were dissolved by stirring overnight at 37 degrees Celsius. The DNA was then precipitated with AutoGen AG00410. The resulting material was washed once with isopropanol and twice with a solution of 70% ethanol. Next, the DNA was dissolved and stored overnight at a temperature of 37 degrees Celsius then stirred. Finally, it was stored at -20 degrees Celsius for samples to be used at a later time, or at 4 degrees Celsius for …show more content…
This assures that DNA will not degrade within 100 years of the processes such as gel electrophoresis, PCR, and AFLP analysis. In comparison to the GenTegra™ DNA tubes, DNAshells® tubes were able to protect the DNA from humidity because they were watertight. GenTegra™ DNA tubes, on the other hand, lost dsDNA while being stored for one week due to humidity. In the DNAshells®, the only factor that caused the DNA to degrade was exposure to high temperature. Also, after 1 week and 1 month at high temperatures during transport, trehalose stabilized the structure of the DNA. This was caused by hydrogen bonding to phosphates and other polar groups with
Show More

Related Documents: Tissue Banking Lab

Stem Cell Research Papers

Stem Cell Research Stem cells are undifferentiated types of cells, which mean that they have the capacity to renew themselves. Their ability to differentiate into other cells is an unlimited process that can be found in all different tissues and organs. Stem cells serve as a “repairing system” for the body, meaning that they can divide without any limit to cure other dead cells. When a stem cell divides, it has the choice to either remain a stem cell or transform into another type of cell. The…

Words 3009 - Pages 13

Designer's Role And Internshipment: An Introduction To Ms. Rinku Kaicker

Acknowledgement I experience great pleasure to convey my profound sense of respect and gratitude to Ms. Rinku Kaicker, design head of wills lifestyle giving us the opportunity to come to this company and get new knowledge and rightful exposure that we students needed. I would like to thank and appreciate the comments and suggestions given to me by Ms. Vatsala Verma for her excellent supervision and encouraging discussions that helped us in having a greater in-look into the project. I would…

Words 7565 - Pages 31

Your Provider Is A Healthtexas Physician

primary care sites are recognized by the National Committee for Quality Assurance (NCQA) as Physician Connections-Patient-Centered Medical Homes (PPC-PCMH) allowing our physicians to coordinate your care seamlessly across our network of specialists, labs, and hospitals in accordance with your specific needs. We appreciate your trust in us and thank you for choosing a HealthTexas physician to meet and monitor your healthcare needs. You can now find a HealthTexas physician with the touch of a button…

Words 5025 - Pages 21

Anatomy Of The United States Medical School: The Commonwealth Medical College

Institutional Issues Anatomy of a New U.S. Medical School: The Commonwealth Medical College Raymond A. Smego, Jr., MD, MPH, Robert M. D’Alessandri, MD, Barry Linger, EdD, Virginia A. Hunt, MUA, James Ryan, John Monnier, Gerald Litwack, PhD, Paul Katz, MD, and Wayne Thompson Abstract In response to the Association of American Medical Colleges’ call for increases in medical school enrollment, several new MD-granting schools have opened in recent years. This article chronicles the development…

Words 6610 - Pages 27