Tryptic Soy Agar Lab Report

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A test tube labeled unknown #9 was handed out by the professor. The first step of identifying the unknown was to immediately gram stain the bacteria. The gram stain results yielded short, gram negative rods with a clustered to random arrangement. Following the gram stain, a streak test was performed on a Tryptic Soy Agar (TSA) plate. After incubation for forty-eight hours, the TSA plate was analyzed to identify a vast amount of pure colonies. The single isolation colonies were circular in form, the elevation was convex, and the margin was entire. Upon the growth of the colonies were pinkish/red dots. A single isolation colony was then chosen to be used throughout the rest of the project. The colony was then inoculated onto a TSA slant in order …show more content…
The plate was identified to have good bacteria growth with fermentation present and was negative for lactose. Since there was bacterial growth, it supports the fact that the bacteria is gram negative. There was a slight blue/black color, which meant that the bacterial species was negative for lactose, but since there was a slight blue/black color present, it indicated that fermentation occurred just not of lactose. To follow up that test, the fermentation of lactose and dextrose was completed by inoculating a dextrose and lactose tube. The lactose tube was negative for fermentation because it had a pink solution, and Durham tube showed that no gas was present. The dextrose tube was positive for fermentation due to a yellow solution being present, and the Durham tube showed that no gas was present. The results were consistent with the EMB plate. At this point, it could be identified that the bacteria belonged to either the genus: Enterobacter, Escherichia, or Klebsiella. The catalase test was then performed with the addition of hydrogen peroxide to a small sample of bacteria, bubbles were immediately present indicating a positive catalase
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