The results of the experiment confirmed that big darters produce more eggs than smaller darters. Along with the study results, the theory of trade-offs between offspring number and size is supported. Based on the results, Turner and Trexler predicted that larvae from larger eggs hatch earlier, feed earlier, cannot drift away so far and cannot disperse far as well. Less dispersion ledsto greater isolation, this then influenced a rapid gene differentiation. To observe the gene flow between the study areas, a genetics background was used. Gene variation influenced a greater amount of gene flow. In other words, larger eggs decreased gene flow, while smaller eggs increased gene flow. The importance of this study was to elaborate on the idea of population interactions and how gene variations are important to the