Unknown Lab Report Essay

Words: 900

Pages: 4

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Name: Danielle

Title: Unknown Lab Report

Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The first procedure that was done was the gram stain. Under a microscope, if the gram stain is purple, the bacterium is gram positive, if the stain is red, it is gram negative. The next test was the fermentation tests for glucose, sucrose and …show more content…
For the gram stain, my organism turned red, meaning that my bacterium was a gram negative and it was also rod shaped. After this test, the possible organisms were: Enterobacter aerogenes, Escherichia coli, Serratia marcescens, Citrobacter freundii, Proteus mirabilis, Pseudomonas aeruginosa, Pseudomonas fluorescens, Salmonella or Shigella. The fermentation tubes were all heterofermented; glucose, sucrose and lactose all turned out to be acid/gas. This narrowed the list down to these possible organisms: Enterobacter aerogenes and Citrobacter freundii. To determine between the two, I did the methyl red test next. The methyl red test was negative, meaning the pH was above 6 and leaving only one organism: Enterobacter aerogenes. To confirm this, the Vogues Proskauer test was performed. The Vogues Proskauer test detects acetoin by oxidation with oxygen in the presence of KOH. These results were positive. The sulfur, indole and motility tests were also confirmations. The bacterium contained no sulfur, did not produce indole and was motile. It

Unknown Lab Report

Identification of Three Unknowns Leah Bacon December 10, 2014 Chemistry 3020 M/W 1:25 - 4:25 William DeBenedetti Abstract Molecular structure can be determined by Fourier Transform infrared spectroscopy [IR, herein] by accurately identifying residues. IR is extremely sensitive to molecular structure because it measures the change in a molecule’s dipole moment due to vibrations caused by light absorbance. In this study, a variety of IR techniques were used to identify the composition of two unknowns by comparing…

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Unknown Lab Report

Identifying Unknown Bacteria in Anonymous Lab Samples Autum Foerster Eastfield College Isolating and Identifying Unknown Bacteria in Anonymous Lab Samples Test tubes were presented with mixed bacteria samples. The samples needed to be identified. Biochemicals help identify bacteria by producing different reactions based on the individual organism. Biochemical tests have been shown to be a reliable method of identifying unknown bacteria. This study will use biochemical tests to identify three unknown bacteria…

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Unknown Carbohydrates Lab Report

The objective of this lab was to find out the four unknown carbohydrates. We first did the Benedict's test we had to put the three different types of saccharides in a test tube which were monosaccharide, disaccharide and polysaccharide. We had to heat them up in hate water, we observed it and only the fourth unknown carbohydrate changed colour with was orange. The first, second and third stayed blue, and the fourth one changed to orange indicating that it was a monosaccharide. After that we did the…

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Unknown Bacteria Lab Report

bacterial colony grown from a sample by using biochemical and cellular tests. These tests determined many of the unknown bacteria’s characteristics. The appearance of strings during the KOH test indicates that the bacteria is gram negative as it means the cell had lysed. This result was not backed up with the results of the test media. The bacteria only grew PEA, which indicate the unknown is gram positive. The conclusion was drawn the the KOH test was a false positive as too much culture was used;…

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Unknown Sample Lab Report

experiment was deliberated to determine if the concentration of select unknown samples exceeded a certain amount that corresponded to the U.S. standard. The concentrations were obtained using a series of calculations and equations via a spectrophotometer, which was the main piece of equipment that provided the percent transmittance. The unknown samples were tested against the diluted stock-AVM solution. It was founded that all selected unknown samples, in fact, did not exceed the U.S. standard of 3.0 mg/L…

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Unknown Lab Report Essay

what physical property was being identified? When testing the powders with water, the physical property of solubility was tested. 2.) Which items appeared to be water soluble? The items that appeared to be water soluble were the baking soda, the unknown substance, cornstarch, and possibly the baking powder (hard to tell because it mostly became bubbles that stayed and didn't go away). 3.) Which items were not water soluble? The items that weren't water soluble were the cream of tartar, detergent…

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Unknown White Compound Lab Report

10/24/2014 Identifying, Confirming, and Synthesizing an Unknown White Compound Group Members: Hayley Ortbals & Christina Navarro Abstract: The main purpose of this lab was to identify an unknown white compound, determine and confirm it’s physical and chemical properties, and synthesize the compound while testing the precipitate formed. The experiments performed for each process consisted of three tests: the solubility test that determined the unknown compound was soluble, anion and cation test which…

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Density Lab Outline Essay

writing a lab report is to provide a blueprint for another scientist to repeat your experiment and get the same results. A COPY OF THIS DOCUMENT incl. the finished PRE-LAB SECTIONis due on FORMAL LAB DAY! Written work should never be copied from other groups. Use your own words! Read skills section in back of text. Make sure you are familiar with using a graduated cylinder and an electronic balance and how to write a lab report (see class website) and how to draw and label diagrams. Required Lab sections-headings…

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Essay about Unknown Esssay

Course Description Laboratory experience with brightfield microscopy; aseptic and pure culture techniques; staining and observation of microorganisms; identification of unknown bacteria; environmental influences and control of microbial growth; antibiotic sensitivity and resistance; bacterial examination of water; medical microbiology. Credit Hours: 2 Prerequisite(s): Credit or concurrent registration in BIOS 350. Laboratory: Science and Engineering Laboratories-East (SEL-E), Room 3083…

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Plot and Sample Xyz Essay

scattered. The objective of the experiment was to determine the unknown concentration of a chromophore, Sample XYZ (XYZ), in an aqueous solution by applying the Beer-Lambert equation. In order to complete this objective, the wavelength of maximum absorbance (λmax) of XYZ and the molar Comment [WO2]: Complete sentences are used to describe the goal of the experiment. Comment [WO3]: If you are going to use an abbreviation in the report, it should be written out in full the first time and defined…

Words 2042 - Pages 9