Four test tubes containing 1 gram of yeast, 8 ml of de-ionized water, and 10 ml of 20% sucrose were placed into four different temperatures for 30 minutes. Test tube 1 was kept at 0oC and produced 0.35 ml of CO2. Test tube 2 was kept at room temperature and produced 15 ml of CO2. After test tube 3 was placed in the 37oC incubator, 15 ml of CO2. 5.0 ml of CO2 were produced by test tube 4 which was placed in an 85oC incubator. Although test tube 3 (37oC) and test tube 2 (room temperature) both produced 15.0 ml of CO2 after 30 mins, test tube 3 is optimal. This can be concluded because it took 15 minutes for test tube 3 to produce 15.0 ml of CO2 and it took 30 minutes for test tube 2 to produce 15.0 ml of