Zebrafish Research Paper

Zebrafish characteristics:

The zebrafish is (a teleost) and belong to the family of Cyprinidae,zebrafhis are the smallest model compare with other mammals that use in lab such as mice. Zebrafish are estimated) less or equal 5 cm) and live in freshwater and locate in north south easia.zebrafish has higher fecundity a single femal can produce more than 200 eggs per week. Zebrafish is a powerful modle to study vertebrate development. Zebrafish and mammals are highly and orthology genes.

Zebrafish :advantage and disadvantage
Advantage:

1. Zebrafish has a small size (less 5 cm) we can see the while animal on slide for histology analysis instaed of using tissue that required a permeation from a patent,and high prolific which can give …show more content…
Zebrafish is a perfect module for druge screen in vivo because of easy to by samply add the chemical into water.
3. Emberyo transpacy lead to detect the pathogen easy that cause systematic infection.
4. Zebrafish genom has been sequenced and found highly conservative with humen genom than other moduels such as fruit fly and nematode.
5. Large genatic screen why ?
6. Easy to identified from the phenotype. (Disease phenotype)
7. Easy to work and study than higher model such as mice.
8. Genetic screening can done easily and with low cost.
9. From the visualization of the embryo zebrafhish is a powerful model to study infection processs

Disadvantage :
1- Zebrafish has shortage of antibody surface protein such as
2- Most of the analsis such as real time done during 8 dayes postfertilization without a mature of Tand B cells than adult fish.
3- Can not establish cell line in vitro experiment.
4- Knockout technology and RNA interference (RNAI) not well establish with Zebrafish module.
5- Incubation temperature for pathogens in Zebrafish between 26c and 29 c, otherwise in human at …show more content…
The host pathogen interaction:
(Streptococcal iniae cause a fatal systemic disease in zebrafhish.in humaen the same pathologies seen in human infection by streptococcus agalactia and s.pneumoniae.) paraphrase it similar to article.
Virulence gene:
Showed that zebrafish has a (vaiable) model by assess the (virulence of mutant microbs).s,pyogenes has ………..the zebrafish was injected with mutant (SIU) gene,and this gene required for iron uptake.and the result showed reduce the severity of the disease into the model (74).Mtsr gene is a regulator that regulate iron acquisition. Mutant in MTSR showed decrease in virulence with zebrafish model.by taking togathere these result, virulence genes can be easily ideanify and vrify by zebrafish model.

Shoed zebrafish modle is used to find virulene mutants,tow muatation was test in s.pyogenes.the first mutation,Rop reglatroy gene was disteed inwhen s,pyogenes inteerdu with the fish,and then result from that disterption is reduced the virulence of the pathogen but with glutathione peroxidase the virulence still high(33).

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