Sodium citrate is a partial reducing agent. Without the addition of glucose in Batch C, the nanoparticles turned more of a pale yellow color due to the same reason in Batch B because glucose, too, is a reducing agent. With the addition of 0.025 g of soluble starch into the silver nitrate solution, the reagent was a deep yellow, or a gold color. The starch stabilized the nanoparticles so that the color was very visible. All nanoparticles aggregated to at least one reagent provided. Batch D would be most supportive of creating 20 nm nanoparticles meaning that the λ max is around 401 nm. Batch D includes all the reagents, glucose and sodium citrate in silver nitrate, plus the addition of 0.025 g of soluble starch. To test silver nanoparticles as an anti-bacterial agent, one could test the application of the nanoparticles on a known bacteria and use an anti-bacterial treatment, like Penicillin, as the positive control and distilled water as a negative control. To do this they would need three plates of agar with E.coli on each plate. On plate #1 would be the bacteria and silver nanoparticles. To make the nanoparticles, one would have to mix and boil silver nitrate, a small amount of glucose, a small amount of sodium citrate, and distilled water until it is a