Biomolecules Lab

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In this lab, we looked to see whether or not four unknown substances contained either carbohydrates, proteins, or lipids. We wanted to discover whether or not those substances, specifically labeled A, B, C, and D, contained those specific biomolecules because as we study more about them, we want to learn when they are present and when they are not. As far as previous knowledge regarding biomolecules goes, I am a former biology student and I vaguely remember learning about lipids (the cell membrane), the production of protein, and carbohydrates (sugars). Although my previous knowledge is somewhat limited, I was definitely interested in what biomolecules we were going to discover in each solution.
To start off, we would mix each solution with a specific test. The first test was to see whether or not the substance contained …show more content…
When the Biuret solution reacts with proteins, it goes from a blue color to make a pink-purple color. So, if any substances were to contain proteins, we would know because they would turn to a pink-purple.
Finally, to figure whether lipids were present in any of the solutions, we mixed each one with Sudan III. The special dye dissolves nonpolar compounds such as lipids and does not in water. Therefore, we can conclude a solution contains lipids when Sudan III dissolves within it.
The first test we did was to see whether or not carbohydrates were present in any of the solutions. For solution A, we found that the yellowish-brown color of the Iodine simply became clearer. As for solution B, the bottom half was clear with some bubbles. At the top, there were lots of bubbles and it turned a darker brown. When solution C was mixed with starch, it completely turned into a blueish black. Thus, starch was present in solution C. Solution D had a red ring at the top with a dark yellow at the bottom when mixed with Iodine. After our tests, we concluded that starch was only present in solution

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