Throughout the years, medical practitioners have treated bacterial diseases with antibiotics. Unknowingly, this has lead to the bacteria to become more and more resistant to the antibiotics to where they do not have the same effect. How this came to be was that the bacteria learned to set up a type of immune system against the effects of the antibiotics. Now when we go to use the same treatments on the bacteria, it doesn’t work as well, sometimes not at all.
The Alliance for the Prudent Use of Antibiotics (APUA) explains how if a large amount of bacteria becomes resistant, then it will become more expensive and difficult to treat the bacterial infections. The APUA goes on to say how this means that people will need more doctor appointments …show more content…
Put back in incubator for further growth
Take out agar containers after another day and make further observations
Collect your new agar containers and decide what bacteria you will put into these, what will be your control group, what antibiotic you will use, and what bacteria will be affected by the antibiotics
Divide the back of the plate with sharpie or some other permanent marker to separate your control group bacteria and the bacteria that will be affected by the antibiotics
Now take the labeled agar plates, your chosen bacteria, and an inoculating loop over to the area where you have the sterilization equipment
Sterilize your inoculating loop *sterilize it before using it on any different bacteria*
Carefully swipe your chosen bacteria from the original agar containers and wipe it thoroughly on the appropriately labeled agar plate
Repeat steps 14-15 for each bacteria you wish to test
Take your antibiotic discs and place it in your desired spot on each agar plate that you just set up
Place the agar plates with your antibiotic discs in the incubator for a day
Take out your agar plates and make observations
Repeat steps 18-19 for at least 2 more days to see the bacteria’s antibiotic