1. Describe why you added the cold hypotonic solution to the pellet of RBCs? (2 marks)
Because the red blood cells burst and release proteins into solution. Hypertonic solution includes less solute then blood does which cause water to need to leave the hypotonic solution and arrive at the region that has a large concentration of solute via osmosis. This is mean that the water will want to leave the intravascular space and enter RBCs, and this cause the extra water in the blood vessels that wants to move into RBC via osmosis where the higher concentration of solute fount there. However, the function of the proteins depends greatly on where the protein is located. For example, RBCs are structures that circulate the body and assist to deliver oxygen from the lungs to the tissues, and carbon dioxide from the tissues to the lungs. However, these cells would not have these competences if they did not have the protein Hemoglobin ( Freeman. 2003). Thus, the protein can be different even inside the cell. For example, in the plasma membrane, a membrane that surrounds the cells and regulates the cells metabolic functions( Freeman. 2003). The protein that could found from vesicles to transport material into and out of the cell ( Alberts. 2014). On the other hand, they could act help for the plasma membrane in the form of actin filaments ( Alberts. 2014). …show more content…
Comment on why it is preferable to store your samples on ice while you are processing them. (1 mark)
it is very essential to maintain the cell sample viable as well as recovery of sample made easy for this. The biological samples should keep in ice chamber to prevent the temperature of sample from contamination of cell and tissues. However, low temperature would help metabolic reactions stops in cell