Scientific, Waltham, USA) was applied. In the second line, the sample was added. The ladder is composed of 15 individual DNA fragments of 20000 bp, 10000 bp, 7000 bp, 5000 bp, 4000 bp, 3000 bp, 2000 bp, 1500 bp, 1000 bp, 700 bp, 500 bp, 400 bp, 300 bp, 200 bp and 75 bp. In each run, the DNA fragments in the sample presented a band between 10000 and 20000 bp.
The characteristics of the two different runs can be found in Table 5.1.. In run 2 more DNA fragments were obtained, the total DNA yield was higher…
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