Lab 4: Histology
Question:
How do the different types of tissue in the body differ from one another?
Hypothesis:
I think that each type of tissue will have significant differences in structure and its own unique qualities compared to other tissue types. If the tissues are compared to one another, then they will each have their own unique qualities and differences in structure. I believe this because each type of tissue has a different function. With cells, the structure reflects its function. I believe this rule will apply to tissues as well since tissues are made up of cells.
Materials:
Parts of the Microscope Simulator (http://www.udel.edu/biology/ketcham/microscope/scope.html)
Virtual Compound Microscope Simulator (http://www.udel.edu/biology/ketcham/microscope/scope.html) http://www.histology-world.com/ http://www.bu.edu/histology/m/index.htm
Textbook (Human Anatomy & Physiology 9th Edition)
Procedures:
1) Start the Parts of the Microscope Simulator by using the provided link in Black Board
2) Correctly assign all parts of the microscope
3) Access the definitions of the parts
4) Use the definitions to provide a description for the components listed by the professor
5) Start the Virtual Compound Microscope Simulator by using the provided link in Black Board
6) Click the “start tour” button to begin the simulation
7) Turn on the light by using the switch at the bottom at the microscope
8) Adjust the rheostat to 10
9) Click on the letter “e” slide
10) Turn the ocular housing to select a lens
11) Select the 4x lens
12) Reposition the slide holder until the specimen is centered over the light
13) Use the coarse focus knob to move the stage as high as possible
14) Switch views so that you are looking through the microscope
15) Adjust the oculars
16) Starting with the stage at the top and at low power adjust coarse focus down until the image appears
17) Use the fine focus knob to improve the clarity of the image
18) Use the XY controls to move the area of interest into the center of the image
19) Use the iris diaphragm to adjust the brightness of the image
20) Switch objectives from 4x to 10x
21) Switch objectives from 10x to 40x
22) Readjust the fine focus and the iris diaphragm
23) Switch objectives from 40x to 100x
24) Readjust the iris diaphragm
25) Switch views so that you are looking at the microscope
26) Change the slides from the “e” letter to the onion root tip
27) Switch to the 4x objective
28) Start the stage at the top position
29) Center the specimen
30) Switch views so that you are looking through the microscope
31) Adjust coarse focus until the image appears
32) Center the image
33) Adjust fine focus to improve the clarity of the image
34) Adjust the iris diaphragm
35) Switch from the 4x objective to the 10x objective
36) Switch from the 10x objective to the 40x objective
37) Adjust the iris diaphragm
38) Switch from the 40x objective to the 100x objective
39) Switch views so that you are looking at the microscope
40) Switch from the onion root tip slide to the bacterial capsule slide
41) Switch to the 4x objective
42) Start the stage at the top position
43) Center the specimen
44) Switch views so that you are looking through the microscope
45) Adjust coarse focus until the image appears
46) Center the image
47) Adjust fine focus
48)