Nt1310 Unit 3 Lab Report

Words: 757

Pages: 4

Open Document

i) To monitor the circulating RV strains/genotypes in environmental samples. Just like the oral polio vaccine (OPV), the two rotavirus vaccines are live-attenuated vaccines and they will always be shed in the environment through feces. In this objective, the monitoring of sewage for both RV wild types and vaccine strains will help in understanding the efficacy of the RV vaccine and will also documenting the epidemiology and seasonality of this strains and also in correlating the strains identified with strains isolated in clinical samples. This is made possible through molecular characterization and typing of environmental RV strains genetically and comparing the data of prototype, vaccines and clinical relevant strains from other regions. ii) To determine monitor the shedding of rotavirus vaccines strains in the environment. Just like poliovirus individuals with acute gastroenteritis associated with RV excretes the high concentration of virus through feces and it is well documented that a infected individual can shed more that 1010 infectious particles per gram of feces. The virus can persist in …show more content…
Since rotavirus is a double stranded RNA virus and segmented it tend to reassort and is able to evolve rapidly via genetic drift, genomic rearrangement, duplications, and deletions of gene sequences, as well as through the zoonotic transmission of strains. In places that are connected by a sewer system or having animal farms rotavirus can be detected in sewage or other environmental samples. e.g in urban areas rotavirus excreted by hospitalized children who are under 5 years of age, the age group mostly affected by this virus ends up into the wastewater. Animals rotavirus coming from farms runoffs can be a source of information of any zoonotic strains that might be in circulation and which has a potential to reassort with the human strains which can. lead emergence of novel RV

Nt1310 Unit 3 Lab Report

The pitch is angled triangular, as this is a preferred method Sinnott (2005). (Eq. 3.4 6) is used to calculate the number of holes per tray: The number of holes per tray above feed stage is 25912, while below feed stage the number of holes is 24793. The choice of hole diameter is usually about 5mm, but an increase in the size of the hole can be considered when fouling causes concern (Sinnott, 2005). The minimum hole size is about twice the size of plate thickness for stainless steel, in this case…

Words 1190 - Pages 5

Nt1310 Unit 3 Lab Report

Chapter Three Design Principles and Implementation 3.1 Overview This chapter deals with the use of the MATLAB software to design the digital-mixer. At first the idea of using the Graphical User Interface for MATLAB was looking promising as it provides a more classic and neat look compare Simulink model. But using the GUI in MATLAB was a very tedious task with all the coding and errors that arose. Therefore, Simulink was chosen as it provides a more direct approach to design the digital mixer…

Words 1229 - Pages 5

Nt1310 Unit 3 Lab Report

of 18 test tubes, placing 3 test tubes in each of the 6 test tube racks; one test tube rack for each variable. This includes 3 test tubes for each variable to find an average and reduce the effect of anomalies making the results more reliable. 2. Then using a pipette extract 4ml of algal solution (Tolypothrix) from the beaker and place in a test tube. Repeat this for all 18 test tubes making sure that the measurement is read from the meniscus to ensure reliability. 3. After this, using a new pipette…

Words 1045 - Pages 5

Nt1310 Unit 3 Lab Report

Output folder filepath: E:\Gwc9 File names: 1) lab5.mxd 2) lab5.shp 3) lab5.img Process Details Exercise 1 NOTES 1. Open up Arcmap and ERAS imaging and add roster layers from the satellite. Add band six then examine after that the person will remove and six and then add band for which is the near infrared band for arc map. In ERAS imaging a person can use multiple windows and open up the same file in different bands this is useful to identify different objects. Q1: Briefly…

Words 591 - Pages 3

Nt1310 Unit 3 Lab Report

Post Lab Questions 1. Based on the amount of yolk, describe the egg type and cleavage pattern of a starfish. Isolecithal, they allow for complete cleavage through the entire egg. They have very little amount of yolk and are uniformly distributed. 2. What is the difference between a'smart' and a'smart'? Is the entire zygote of a starfish involved in early cleavage? Explain the adage of the adage. ? no it is holoblastic (the division of the entire egg into blastomeres). This allows for complete cleavage…

Words 402 - Pages 2

Nt1310 Unit 3 Lab Report

Basics of a-Si:H/uc-Si:H tandem solar cells Figure 1.2 shows the scheme of the one of the possible configuration for the thin-film tandem solar cell, which consists out of three blocks: the upper thin-film part, the thick layer of Silicon wafer and the bottom thin-film part, which are built in stack. Figure 1.2. The schematic view of the HIT solar cell According to Schockley the energy of photons, which exceeds the band gap is lost due to the thermalization of the charge carriers to the band edges…

Words 1025 - Pages 5

Nt1310 Unit 3 Lab Report

flow rate. The fluid flows through hose pipes (0.5in.) by a pump (0.5 hp and 30-40 m head) from the tank to the radiator at the flow rate range 500-550 LPM. The total volume of the circulating fluid is 3lts and constant in all the experimental steps. 3.…

Words 1051 - Pages 5

Nt1310 Unit 3 Lab Report

This manuscript developed a HIF-1α stabilizing wound dressings by incorporating dimethyloxalylglycine (DMOG) in electrospun core-shell nanofibers. The authors showed the advantages of the material fabricated by the characterization, the release test, cell culture experiment and animal experiments. However, the reviewer thought that the detailed description of electrospinning process is incomplete. Following the precise analysis of the structure and the amounts of drug incorporated in fibers, the…

Words 502 - Pages 3

Nt1310 Unit 3 Lab Report

Diaphragm h. Coarse focusing knob i. Illuminator j. Base ~~5. What type of microscope has primarily been used in this lab? (1 point) The compound light microscope was used in this lab. ~~6. State the function of the following parts of the microscope: (3 points) A. mechanical stage- This is the area where a specimen is placed on the microscope for viewing. B. ocular lens- This is used to magnify the image that has been formed…

Words 939 - Pages 4

Nt1310 Unit 3 Lab Report

3.1.5.3. Coverslip culture technique Coverslip culture technique was used for reviewing the morphological characteristics of the marine actinomycetes isolates (Pridham et al., 1958). Sterile glass cover slips were inserted at an angle of 450 into solidified SCA medium in petri plate. A loopful of inoculum of each isolate was streaked laterally to the line where the coverslip come across the agar and then the plates were inoculated at room temperature (28±200C) for 7 days. After the development of…

Words 688 - Pages 3