Oxygen and Test Tube Essays

Submitted By nukuglyass
Words: 1464
Pages: 6
Open Document
Sugar Fermentation in Yeast

Yeast are able to metabolize some foods, but not others. In order for an organism to make use of a potential source of food, it must be capable of transporting the food into its cells. It must also have the proper enzymes capable of breaking the food’s chemical bonds in a useful way. Sugars are vital to all living organisms. Yeast are capable of using some, but not all sugars as a food source. Yeast can metabolize sugar in two ways, aerobically, with the aid of oxygen, or anaerobically, without oxygen.

In this lab, you will try to determine whether yeast are capable of metabolizing a variety of sugars. Although aerobic fermentation of sugar is much more efficient, in this experiment we will have yeast ferment sugars anaerobically. When the yeast respire aerobically, oxygen gas is consumed at the same rate that CO2 is produced—there would be no change in the gas pressure in the test tube. When yeast ferment the sugars anaerobically, however, CO2 production will cause a change in the pressure of a closed test tube, since no oxygen is being consumed. We can use this pressure change to monitor the respiration rate and metabolic activity of the organism. A Gas Pressure Sensor will be used to monitor the fermentation of sugar.

The fermentation of glucose can be described by the following equation:

C6H12O6 [pic] 2 CH3CH2OH + 2 CO2 + energy glucose ethanol carbon dioxide

Note that alcohol is a byproduct of this fermentation.

[pic]

Figure 1

MATERIALS

|LABPRO OR CBL 2 INTERFACE |18 X 150 MM TEST TUBE |
|TI GRAPHING CALCULATOR |1-L BEAKER (FOR WATER BATH) |
|DATAMATE PROGRAM |BASTING BULB OR BERAL PIPETTE |
|VERNIER GAS PRESSURE SENSOR |HOT AND COLD WATER |
|GRAPHICAL ANALYSIS (OPTIONAL) |TEST TUBE RACK |
|RING STAND |UTILITY CLAMP |
|RUBBER-STOPPER ASSEMBLY |THERMOMETER |
|5% GLUCOSE, SUCROSE, LACTOSE, AND ONE OTHER SUGAR SOLUTION |YEAST SUSPENSION |
| |VEGETABLE OIL IN DROPPER BOTTLE |

PROCEDURE

[pic]

Figure 2
1. Connect the plastic tubing to the valve on the Gas Pressure Sensor. If your sensor has a blue plastic valve on it, place the valve in the position shown in Figure 2.

2. Plug the Gas Pressure Sensor into Channel 1 of the LabPro or CBL 2 interface. Use the link cable to connect the TI Graphing Calculator to the interface. Firmly press in the cable ends.

3. Turn on the calculator and start the DATAMATE program. Press [pic] to reset the program.

4. Set up the calculator and interface for a Gas Pressure Sensor.

a. If the calculator displays PRESS(KPA) in CH 1, proceed directly to Step 5. If it does not, continue with this step to set up your sensor manually. b. Select SETUP from the main screen. c. Press [pic] to select CH 1. d. Select PRESSURE from the SELECT SENSOR menu. e. Select the correct pressure sensor from the PRESSURE menu. f. Select the calibration listing for (KPA). g. Select OK to return to the main screen.

5. Prepare a water bath for the yeast. A water bath is simply a large beaker of water at a certain temperature. This ensures that the yeast will
Show More

Related Documents: Oxygen and Test Tube Essays

Enzymes Under Different Temperature Conditions Lab Report

took 4 empty test tubes and placed it on a tube rack. Then filled each test tube with 5 ml of 3.0 % Hydrogen peroxide (H2O2) and 3 ml of water (H20); and to make sure not to confuse the test tubes, labeled each test tube by using a wax pencil, with the initials of one member of the group and with test tube labeled number 1, 2, 3, and 4. First, placed test tube 3 in the water bath, which has been prepared on the side bench in order for it to reach 37 degree Celsius. With test tube 1, in order to…

Words 1086 - Pages 5

Oxygen and Aerobic Respiration Essay

significantly more oxygen than nongerminating, or heat killed, peas, as anticipated by the students performing the lab. Aerobic respiration was necessary for the germinating peas because the oxygen released energy from the peas’ glucose to give fuel for germination. The major conclusion reached by the students was that plants require not only carbon dioxide but also oxygen to live, thrive, and reproduce. The students who performed the experiment already knew that aerobic respiration required oxygen, but the…

Words 832 - Pages 4

Rate Of Oxygen Consumption During Respiration

rate of oxygen consumption by germinating peas at varying times during the germination process. Objective: The objective of this lab was to observe the rate of oxygen consumption of germinating peas at four times in the germinating process, 0 hours, 18 hours, 46 hours and 65 hours, and how the varying times of germination would affect the peas’ oxygen consumption during respiration. Null Hypothesis: The length at which the peas were left to germinate will not affect the peas’ rate of oxygen consumption…

Words 1292 - Pages 6

Essay about AP Investigation Lab #13 Enzyme Activity

got, the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased about 10% a minute until it sort of equilibrated at 4 minutes and didn’t change to the fifth minute mark. If we were to change anything we did in the experiment, we would make our comparisons to the chart more precise. Overall we thought it…

Words 1912 - Pages 8

Lab 1 The Determination of R Essay

apparatus involving a test tube and large beaker with rubber stoppers and tubing and equalized atmospheric pressure, the test tube contained approximately 0.02 g of MnO2 and 0.2972 g of KClO3 to yield 0.1322 g of oxygen and 80.0 mL of O2 gas. Because both equations and error analysis needed to be utilized to provide a lesser uncertainty of R, the determination was around 0.06434 L*atm/mol*K. Introduction In using the apparatus to determine the proper value of “R”, the test tube connected will be weighed…

Words 1157 - Pages 5

Report On Manganese Oxide

up to create oxygen at a faster rate. Equipment: Manganese (IV) oxide – The catalyst used to test the hypothesis. Conical flask – Used to contain the hydrogen peroxide and manganese (IV) oxide . Timer – To time how long it takes for the water to come out of the tube due to oxygen entering. Hydrogen peroxide – The solution used to test the hypothesis. Bung – Used to stop gas escaping from the flask. Delivery tube – To collect the oxygen that was produced. Water trough – To collect oxygen over water…

Words 921 - Pages 4

Fermintation Lab Essay examples

that it is done without O2. The equation for fermentation is C6H12O6⇒2C2H5OH+2ATP. This fact makes fermentation an anaerobic function, because it doesn't use Oxygen. Cellular respiration is an aerobic function, because oxygen is necessary for it to be carried out. many bacteria can use fermentation, and for it to happen, there has to be no oxygen, and warmer temperatures speed up the reaction. The type of fermentation in this lab is called alcohol fermentation, and yeast is used to measure how much…

Words 639 - Pages 3

Macromolecules Lab Report

which the change in color occurred, and could lead to an incorrect conclusion regarding the amount of macromolecules present in a certain food sample. Additionally, it’s possible to have used the incorrect substance for certain tests, such as using starch for the Biuret Test. If this were to occur, the results would be compromised, since no color change would be present. Citation Laying the Foundation in Biology, 2004. McMush: Testing for the Presence of…

Words 989 - Pages 4

Biology Controlled Assessment Photosynthesis Year 9 Final Essay

Bibliography 2 Intro In this experiment we are going to be focusing on photosynthesis and the amount of oxygen bubbles produced as the amount of light intensity increases. What is photosynthesis and its importance? Photosynthesis is the process in which an organism uses sunlight to absorb the nutrients such as carbon dioxide and water. Generally the organism creates a by-product, oxygen. The importance of photosynthesis is that without it, living organisms such as green plants wouldn’t have any…

Words 2940 - Pages 12

Hydrate Research Paper

holds on to a water molecule. An anhydrate is the molecule after it has been removed from the water molecule. The test tube must be cooled before finding its mass because a hot substance has a lower density than a cool substance, therefore, when the formula is used, M=DV, the hotter substance will have a lower mass, and the cool substance will have a higher mass. In addition, a hot test tube could damage the scale, as it is electronic. The mass of the anhydrous salt must be measured immediately on cooling…

Words 432 - Pages 2