Spinach Lab Report

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Spinach leaves (10.0 g) were weighed on a weighing boat. Sand (15.0 g) was weighed and added to the mortar of spinach leaves. The spinach leaves and sand were grinded into a pasty mixture. The paste was transferred through a powder funnel into a 250-mL Erlenmeyer flask. Acetone (50. 0 mL) was used to wash the mortar, pestle, and funnel, and then place the wash into the 250-mL Erlenmeyer flask. Repeat the washing step with a second addition of 50.0 mL portion of acetone. Vigorously swirl the mixture for 2 to 3 minutes. Allow the solids to settle at the bottom, and carefully decant the supernatant into a 500-mL round bottom flask. Acetone (100 mL) was added to the solid in the 250-mL Erlenmeyer flask, so extraction process can occur again. …show more content…
Measured out (40 mL) of silica gel so it can be transferred to a 150-mL beaker. To create a slurry, a sufficient amount of 20% ethyl acetate in hexane was added. Once the slurry was made then it was added to the column. Next, a 250-mL beaker was placed under the column so the solvent can drain when the stopcock was opened. The effluent from the column was used to transfer the remaining silica gel from the 150-mL beaker. After the column was packed, drain the solvent to a level of 0.5 cm above the silica gel. The pigment-silica gel mixture was carefully poured onto the column while using a powder funnel. Sand (0.5 cm) was immediately added to the top of the column. A sufficient amount of solvent was added to the empty space above silica gel, so the column is two-thirds full. The stopcock was open to allow the eluent to drain. Started collecting colored fractions in an Erlenmeyer flasks. Once the yellow band was eluted from the column, add 40% ethyl acetate in hexane in column until it is two-thirds full. Collected the colored bands as they elute from the column, and increase the ethyl acetate as necessary to maintain the movement of the bands. The polarity differences between the compounds determined the four colored components. Each sample of the colored bands was place under UV radiation. The observations of each sample was observed. The color fraction and colorless or excess solvent was disposed in their proper
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