Spinach Photosynthesis Lab

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Abstract:
The closer the light was to the spinach leaf the more O2 that was created which increased the rate of photosynthesis in the spinach leaf. We addressed this problem by using the same light intensity but at different distances from the plant seeing which distance had the most O2 intake for the plant. Our key observation was light intensity that was being put on the spinach leaf. After going through our trials we have concluded that out of our results they support our hypothesis and with the focused light source at the third distance (15 cm; 32500 Lux) the most O2 was taken in by the spinach leaf.
Introduction:
Photosynthesis is the conversion of light energy to chemical energy that is stored in sugars or other organic compounds which occurs in plants, algae and certain prokaryotes. During photosynthesis plants use the Calvin Cycle which is the second of two major stages in photosynthesis, involving fixation of atmospheric CO2 and …show more content…
Then using your long forceps take a piece of spinach from the leaf blade and place the leaf stalk inside of the micro centrifuge tube, and once you have your leaf inside then grab the tube with your forceps and lower it into the test tube. Make sure that the leaf fits properly into the tube (the leaf may be modified to fit properly inside the tube), and make sure that the underside of the leaf (side lightest in color) does not come in contact with the wall. Finally insert the test tube into the hole on top of the water bath and make sure it is in deep enough so that only 2 cm of the tube is sticking out of the water bath on top. Then take the oxygen sensor and insert it into the opening of the test tube so that it is tightly sealed. With the shoebox make sure your water bath is placed inside of the half shoebox so that the there is no light coming in from any other
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