Unit 4 Protein Chemistry And Enzymes Lab

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Name: Loan Truong/ ID: 01519951 / Thursday Lab
Pre-Lab # 4: Protein Chemistry and Enzymes

Title: Protein Chemistry and Enzymes
Purpose: protein have many functions in the body such as: structural support, work in cell membrane, work as enzymes to help run about every chemical reaction that need to occur in the body
Procedure:

Denaturation of Albumin: there are 4 test tubes: 1-control, 2-heat, 3-acid, 4-base. Mark 3cm in test tubes 1 and 2. Mark 4cm in test tubes 3 and 4. The 1 test tube will be your control. This is a solution of albumin looks like when the protein has not been denatured. Place test tube 2 in the boiling water for 5 minutes, add nitric acid in test tube 3, add KOH to test tube 4. Then compare the appearance of test tube 2,3, and 4 with test tube 1.

Enzyme function:

First experiment: the experiment needs four 100 ml beakers, 4 glass pipets, 8 clean test tubes, a bottle of iodine and 4 spot plates from the …show more content…
Place 5ml of each pH starch solution in 4 beakers that notes as pH3, pH5, pH7, and pH9. Label 8 wells on your spot plates as 1 min, 2 min, 3 min, 4 min, 5 min, 6 min, 7 min, and 14 min. Labels two wells as “control water”, and “control starch” in one of your four spot plates. Then place 2-3 drops of iodine in each of your labeled well, and add 2-3 drops of water to the “water control” label. Add 2-3 drops of any starch solution to the well label “control starch”. Ask professor over to add the enzymes, amylase to each of your starch solutions. Gently agitate and note the time mixing.
Then add 2-3 drops of any starch solution to the label well at the time that indicated. Due to we have 4 starch solution, so we need 4 people in a group working at the same time with different spot plates to avoid confusion. Then we compare the color of the solution in the wells over 14 minutes, and two of your controls. Note your notice, and thinking about the optimal pH for fungal
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