Some are Environmental Protection Agency (EPA), Occupational Safety and Health Administration (OSHA), and Body Substance Isolation (BSI). The Environmental Protection Agency controls sterilization and disinfection. Both are used by the dental assistant. The dental assistant uses sterilization using an autoclave. An autoclave kills bacterial spores. Instruments are put in an autoclave to sterilize so the next patient doesn't get sick or diseases. An dental assistant uses disinfection with an ultrasonic. The ultrasonic cleans and removes debris such as blood and saliva. The ultrasonic uses sound waves to do this. The Occupational Safety and Health Administration gives regulations to protect employees and employers. Also, the body substance isolation controls PPE and barriers. PPE is personal protective equipment. PPE includes scrubs,goggles, and gloves. The scrubs must be high neck, and cuffed wrists. Barriers are barrier tape also know as cover film. Cover film is put on light switches and drawer handles. This is because while in patient care the assistant might need to go to a drawer. The cover film prevents