E. Coli Lab

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The purpose of the series of E. coli labs was to isolate its DNA and analyze it through gel electrophoresis. Although I was unable to isolate any E. Coli DNA, my partner was able to; thus, any comparisons use her DNA. Furthermore, we ran our salmon sperm DNA on the gel as a visualizer for large amounts of DNA on the gel. Also, there was a pGLO plasmid as a positive control, and a ladder, to compare base pairs on the bands of DNA. On the gel, I can see three things of importance. First, the salmon sperm DNA shows up in bright, large streaks on the gel. Next, the band of JM109 DNA (the strand of E. Coli used in the experiment) was high up, but light. Finally, the only visible band of E. coli is roughly aligned with the top band of the ladder. For gel electrophoresis, brighter bands equates to a higher …show more content…
This can be attributed to both technical and procedural errors that occurred during the experiment. Early on in the series of labs, we had to spool the E. coli DNA, which had us wipe off excess ethanol off of the spooled DNA on paper towels. In doing so, it is plausible that some DNA could have been wiped off along with the ethanol. No DNA here means that there would be no DNA on the gel. A remedy for this problem would be to have a different technique to remove the ethanol. Moreover, the agarose gel that we made had a low concentration of 0.7%; therefore, it hindered the movement of any smaller strands of DNA. Lower concentrations of agarose gel are better for allowing large strands of DNA to move through. Additionally, experiments that run only one trial are less trustworthy, since results differ between trials. Adding more trials could allow for a higher chance of getting results that you want; in this case, getting DNA on the gel. Some possible extra trials could be growing more colonies of E. coli, or making multiple gels to collect a variety of

E. Coli Lab Report

Discussion: The antimicrobial substance that was most effective in inhibiting the growth of E.coli was TE. The inhibition zone created by the antibiotic was 31.75mm, this created a bacteriostatic effect because the antibiotic was able to destroy the pathogenic growth of the microbes. The second most effective antimicrobial on E.coli was AM, this created an inhibition zone of 27.94mm. This also showed signs of bacteriostatic effect because of the growth inhibition. The third most effective antimicrobial…

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Unit 6 Research Assignment

Quiz 6 Research Assignment My unknown bacteria was E. Coli which is a aerobe, mixed acid fermenting, gram negative bacteria. 3 biochemical tests I could have done during the process of identifying my unknown bacteria is the nitrate reductase test, ONPG Test and the MUG test. The nitrate reduction test was one test we learned about in lab but did not use during the unknown identification process. This test helps identify a bacteria that produces the nitrate reductase enzyme which hydrolyze nitrate…

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Essay on Molecular Techniques

perform the investigation well. This experiment is meant to practice these techniques, and has exercises in micro-pipetting, aseptic technique, and plate streaking. These three applications of molecular techniques are invaluable skills to have in the lab and can be carried out in practice to gain experience and also provide valid feedback. Method The first exercise, pipet techniques, involves the use of multiple variations of micropipettes devices and their respective tip. After going over proper…

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My name is not real Essay

you learned and how the lab can be improved References All literature sources used are listed here Same Some of your labs may have extensive handouts that allow room for data and questions. In those cases, omit the introduction, methods, and results (rational for methods can go into the discussion). Answer ALL questions in the handouts. Tyler White 10/8/13 Bacteria Lab Observing Bacterial Transformation by Introducing PGreen Protein Abstract: E. Coli was collected and transformed…

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Micro Lab Essay

Rebekah Ziesmer - RT 4 March 2013 Micro 233 - Dias Selective & Differential Media Lab Report 1) Hypothesis/Objective: 2) Results: | Organisms | | Mannitol Salt Agar (MSA) | MacConkey’s Agar | Selective/Differential? | 1. | E. Coli | Growth: | No growth | Growth | MSA inhibited growth | | | Reaction: | | Circular colonies | MacConkey’s promoted growth | 2. | Enterobacter aerogenes | Growth: | No growth | Growth | MacConkey’s promoted growth | | | Reaction: | | Circular…

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The Effects Of The Cell Cycle

Morgan Fenoseff Bio 159 3/9/15 E. Coli Gowth INTRO In order for any cell to grow, it needs a source of nutrients; also known as energy. The process in which cells grow and multiply is known as the cell cycle. Once energy is attained within the cell, the cell wall inflates, and the cell begins to expand in size. Similarly, bacteria populations also go through phases of growth. There are four main stages of bacteria growth. (Earl 2014)…

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Pglo Lab Report

Purpose The overall purpose of the pGLO lab was to transform E. Coli bacteria by adding materials such as luria broth, ampicillin, plasmid, and transformation solution. Transformation is a process in which one strain of bacteria is changed by a gene or multiple genes from another strain of bacteria. Procedures The first thing done for this lab was prepping the plates. Students labelled the plates, and then teachers put in agar agar in each plate, adding either pGLO plasmid, ampicillin, or arabinose…

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Essay about BWilkins Lab04

Bailey Wilkins Lab 03: Bacteriology Results: During this experiment we conducted gram stains on four different bacteria, and also tested which antiseptics were most effective on each bacteria. The bacteria that were tested are Bacillus megaterium, Escherichia coli, Micrococcus leutus, and Serratia marcescens. The gram stain results showed that the Bacillus M. bacterium is gram positive, and has the shape of a rod. When observed under the microscope, it was seen that it was in fact purple meaning…

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Summary Of E. Coli Infection At The Lookout

E. Coli Infection at The Lookout As a communications lead at The Lookout, I know a public health scandal has significantly damaged the company's reputation. Customers have become ill from eating at several of our locations, where lettuce and spinach were infected with E. coli. The company is implementing strategies to improve restaurant sanitation, including more thorough training for new and current employees. We must keep our customers abreast of the situation to maintain a positive relationship…

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assignment 1 Essay examples

parameters (i.e., person, time, place, ethnicity, and gender) used in the compilation of data into the information summative. An outbreak of E-coli has occurred in Good Health Hospital, this has become a major problem since the outbreak occurred in Ward 10 on the second floor. It appears that there are six (6) cases of the disease associated with the E-Coli outbreak. The administrator of Good Health Hospital need to be in constant communication with the CDC to help determine what protocol(s) we…

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