Pglo Lab Report

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Purpose The overall purpose of the pGLO lab was to transform E. Coli bacteria by adding materials such as luria broth, ampicillin, plasmid, and transformation solution. Transformation is a process in which one strain of bacteria is changed by a gene or multiple genes from another strain of bacteria.
Procedures
The first thing done for this lab was prepping the plates. Students labelled the plates, and then teachers put in agar agar in each plate, adding either pGLO plasmid, ampicillin, or arabinose to different ones. Then, taking starter plates, students added a liquid that would cause bacteria to develop, and used a sterile loop to spread it. On the day of the lab, groups of students went to their stations and started off by getting a cup …show more content…
19. The normal E. Coli had more development.
20. Since it had plasmid and ampicillin, it had some saller colonies grow, the -DNA did not have plasmid.
21. One had plasmid and ampicillin while the other did not. So, one had growth and one did not.
22. The arabinose one glowed, while the other did not, so the arabinose causes the bacteria to glow.
23. It had glowing and larger colonies.
24. Protein.
In my opinion, everything from the E. Coli did not alter, besides shape and size. Some bacterium were larger, and some were smaller. The results obtained occurred due to perfectly following the lab instructions that were given and making sure to put in the correct amount of liquids in. The bacteria/protein was glowing due to the arabinose, and its carbon and energy. It makes the GFP gene activate and grow glowing bacteria. Transforming the bacteria was successful, since the bacteria grew in the correct plates and glowed in the +DNA/LB/AMP/ARA plate. The arabinose and UV light are important factors that help see the glowing. The UV light causes the GFP to reverberate, and turn green when under the presence of blue light. When an organism is able to turn on or off any particular genes in certain conditions, some advantages would include not wasting certain things such as proteins and not overproducing
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