+ Pglo Lab Report

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Before collecting data and analyzing your results answer the following questions.

1. On which of the plates would you expect to find bacteria most like the original non-transformed E. coli colonies you initially observed? Explain your predictions.

Plates 3 and 4 should contain bacteria that are most like the original non-transformed E. coli colonies. This is because these plates serve as control plates. They do not contain the pGLO plasmid that causes genetic mutations or transformations. Plate 3 should not contain any bacteria because it has ampicillin, which prevents bacteria growth, but plate 4 should contain bacteria because it does not contain ampicillin.

2. If there are any genetically transformed bacterial cells, on which plate(s) …show more content…
Carefully observe and draw what you see on each of the four plates. Put your drawings in the data table in the column on the right. Record your data to allow you to compare observations of the “+ pGLO” cells with your observations for the non-transformed
E. coli. Write down the following observations for each plate.

See image below question 4.

2. How much bacterial growth do you see on each plate, relatively speaking?

Plates 1 and 2, which contained the pGLO plasmid, contained a large amount of bacterial growth. Plates 3 and 4, which served as the control plates and did not contain the pGLO plasmid, differed in that Plate 3 did not contain much evidence of bacteria growth and plate 4 showed signs of some bacterial growth. This makes sense because plate 3 contained ampicillin, which prevents bacterial growth, while plate 4 did not. Relatively speaking, plates 1 and 2 had a lot of bacterial growth, plate 3 had little to no bacterial growth, and plate 4 had noticeable bacterial growth.

3. What color are the bacteria?

Plates 1, 2, and 3 showed signs of bacterial growth. Plates 1 and 3 had clear bacteria. Plate 2 had green, fluorescent bacteria. This makes sense because plate 2 contained arabinose, allowing the bacteria to produce Green Fluorescent Protein …show more content…
Do you observe some E. coli growing on the LB plate that does not contain ampicillin or arabinose? Yes. Plate 4 shows bacterial growth and does not contain ampicillin or arabinose.

1. From your results, can you tell if these bacteria are ampicillin resistant by looking at them on the LB plate? Explain your answer.
The results show that plates 1 and 2 are ampicillin resistant because bacteria continued to grow with the +pGLO plasmid inside the plate. This indicates that the bacteria in plates 1 and 2 are genetically transformed, because they continued to grow even in the presence of ampicillin. It is important to note that the experiment cannot fully prove that the bacteria are ampicillin resistant. These bacteria were in a controlled environment, including controlled temperature and sealed plates. The bacteria would need to be tested in a multitude of environments several times before they could be identified as ampicillin resistant.

2. How would you change the bacteria’s environment—the plate they are growing on—to best tell if they are ampicillin
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