Forensic DNA Fingerprinting Paper

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Forensic DNA fingerprinting can be used to analyze and match evidence in criminal cases. Restriction fragment length polymorphism (RFLP) is a technique that uses restriction enzymes to cut DNA at specific restriction sites, making fragments of the DNA. The band patterns these DNA fragments make are what we call the “DNA fingerprint” or “DNA profile”. In this study we used ENZ, an enzyme mixture of PstI and EcoRI, to digest or cut each DNA sample (from the crime scene and five suspects) into fragments and used gel electrophoresis to produce the DNA profile to determine if there is a match and which suspect is the match. The band pattern for each sample should be different unless the DNA sequence is the same; thus, eliminating suspects who do not match. Results showed that bands from S3 matched bands from CS, placing that suspect at the crime scene. All other suspects had unique band patterns, eliminating those …show more content…
Jeffreys saw that portions of DNA sequence vary among individuals. (Abullah, et al., 2014). Of the millions of nucleotides in our DNA, approximately 1 in 1000 is a site of variation, or polymorphism, in the population (Evett, Werrett, Gill, & Buckleton, 1989). With the exception of identical twins, each individuals’ DNA sequence is different, with a one in sixty-four billion chance for comparable DNA (comparable DNA has similarities but not identical) (Claridge, 2015). Cutting DNA with different sequences into fragments with an enzyme would produce unique band patterns in different DNA. These different band patterns make up the “fingerprint” of a person’s DNA and much like a fingerprint is unique to each individual. Digesting DNA with restriction enzymes, then separating the fragments with gel electrophoresis can be useful in criminal investigations, paternity testing, environmental sampling, and ancestry analysis, among many other uses (Brown, 2011, p.