Guaiacol Lab

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The correlation Turnip extract and Guaiacol have on reaction rates Azriel Molina 3/14/24 BIOL-1406-K-SPRING-2024 J. Trujillo

Abstract: The following information is a report on the results obtained for labs five and six. Lab five was composed of two activities: (1) Effect of Enzyme Concentration on Reaction Rate and (2) Effect of Substrate concentration on Reaction Rate. Activity one tested three distinct types of enzyme concentrations, low, medium, and high enzyme concentrations. Within the different enzyme concentrations were a control, substrate, and enzyme group. The following substances were also used in the experiment, Guaiacol, Turnip Extract, Peroxide, and Distilled water. Activity two used three different amounts of the enzyme concentration being 0.5 ml, 1.0 ml, …show more content…
Measuring the reaction rate with the substrate it was clear that the medium concentration had the highest reaction rate in which our group concluded that a specific amount was better than a higher, this being evident due to the highest amount of substrate having the lowest reaction rate. The results for lab six proved that enzymes function best at a desired temperature. Enzymes function best at 37 degrees Celsius followed by 4 degrees Celsius and 23 degrees Celsius and 60 degrees Celsius being the lowest reaction rate due to the enzyme not being able to function in that type of environment. Activity two results lead us to believe the effects of inhibitors could vary, zero drops had the highest reaction rate, and the number of drops would not really affect the reaction rate in the future, but keep it constant. Five drops caused the reaction rate to act quickly but be constant, and the one drop was the same but at a much slower rate. With this new knowledge, we can learn more about chemical reactions and what may cause a change in reaction

The Effect Of Ph On The Rate Of An Enzyme Reaction

The Effect of pH on the Rate of an Enzyme Reaction Purpose/Problem The problem being investigated in this lab is the effects of salt concentration, pH, temperature, and inhibitors on the rate of an enzyme reaction. Background Information If the salt concentration of a solution is very low or zero, the charged amino acid side chains of the enzyme molecules would stick together and cause the enzyme to denature and be an inactive substance. The pH scale ranges from 0 to 14. When pH is between 0…

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How Different Ph Solutions React To Different Products

In this experiment we tested how different PH solutions react to different products. Through different trials we used the spectrophotometer to measure the absorbance of the reactions between guaiacol, H2O2, and the enzyme peroxidase. An enzyme is a catalyst that speeds up an enzyme, which lowers the activation energy. We use these to see how they influence the reaction rate of turnip peroxidase. The reactions will differ as we change the Ph. solutions. Before the experiment, we put on our safety…

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Enzyme Lab

A piece of tape has ‘tube #4’ written on it, it was then placed on an empty tube. The liquids were converted from ml to μl. Guaiacol was converted from 0.1 ml to 100 μl and was extracted using a 200 μl pipette that was adjusted to 100 μl. Guaiacol was then placed in tube #4. The tip from the 200 μl was disposed of and a new one was placed on it. H202 was converted from 0.2 ml to 200 μl which was also extracted by the 200 μl pipette.…

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Enzyme Activity Lab Report

In this lab we tested the effect of temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance, which gave us a lower…

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Enzyme and Hydrogen Peroxide Essay

Biology lab Introduction: In this lab, “Determining the Properties of an Enzyme”, the objectives were to observe the activity of an enzyme in a tissue extract using a spectrophotometer, and to test whether the amount of the enzyme influences the rate of the reaction, whether the temperature of the solution influences the activity of an enzyme, or whether the pH of the solution influences the activity of the enzyme. Thousands of chemical reactions occur in a cell each minute, these reactions are…

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Turnip Experiment

These lab explorations identified the rate of reaction and effect of substrate and enzyme concentration while observing the reactions of enzyme and substrate concentrations in different temperatures. I hypothesized that the solution with higher temperature would have a faster reaction rate. The enzyme source was the turnip extract. Guaiacol serves as the color reagent in this experiment to help absorb light. The enzymes concentrations used were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract. The substrate…

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Peroxidase Lab Report

My hypothesis in terms of the pH outcome was correct; as the pH balance increased, the overall rate of the peroxidase absorbance levels increased alongside it. It seemed like common sense to make this prediction since pH levels affect us every single day, so why wouldn’t they affect an enzyme reaction? We are surrounded by pH balances on a constant basis, everything we either eat or drink has one! If something has a pH balance less than 7, then it is considered acidic - some of these examples would…

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Essay about AP Investigation Lab #13 Enzyme Activity

Background Information Part 1 In the first part of the enzyme lab, we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together, we observed and saw a change in the color of the substance. The darker and more brown the substance got, the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased…

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Essay on Horseradish Peroxidase

Effects of Temperature, PH, boiling and concentration on Horseradish Peroxidase ABSTRACT The purpose of this report is to find out the effect of change in the Temperature, PH, boiling, concentration in peroxidase activity. Peroxidase is an enzyme that converts toxic hydrogen peroxide (H2O2) into water and another harmless compound. In this experiment we use, turnips and horseradish roots which are rich in the peroxidase to study the activity of this enzyme. The activity of peroxidase with change…

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